Takahashi Yusuke, Tanaka Maki, Murai Ryosei, Kuribayashi Kageaki, Kobayashi Daisuke, Yanagihara Nozomi, Watanabe Naoki
Department of Clinical Laboratory Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan.
Phytother Res. 2014 Oct;28(10):1513-9. doi: 10.1002/ptr.5157. Epub 2014 May 2.
Evidences are accumulating that extract of Acanthopanax senticosus Harms (ASH; syn Eleutherococcus senticosus [Rupr. & Maxim.] Maxim), a shrub native to Northeastern Asia, has antiinflammatory effects. In this study, we examined prophylactic and therapeutic effects of ASH extract (ASHE) on rheumatoid arthritis using collagen-induced arthritis (CIA) mouse model. Acanthopanax senticosus Harms extract was administered before the onset of arthritis in the prophylaxis model. In the therapeutic model, ASHE was administered after the onset of arthritis with or without anti-TNF-α antibody. The ASHE treatment showed efficacy before onset of CIA but there was no effect after CIA was established. The ASHE treatment delayed the onset and decreased severity of CIA. In vitro examinations showed that ASHE is an antioxidant and that ASHE suppresses TNF-α and interleukin-6 production in human peripheral blood mononuclear cells. The combination therapy with ASHE and anti-TNF-α antibody reduced the severity of arthritis compared with anti-TNF-α antibody alone. The present study shows that ASHE has prophylactic effect against CIA and support therapeutic effect of anti-TNF-α antibody.
越来越多的证据表明，刺五加（ASH；同义词西伯利亚刺五加[Rupr. & Maxim.] Maxim）——一种原产于亚洲东北部的灌木——的提取物具有抗炎作用。在本研究中，我们使用胶原诱导性关节炎（CIA）小鼠模型研究了刺五加提取物（ASHE）对类风湿性关节炎的预防和治疗作用。在预防模型中，在关节炎发作前给予刺五加提取物。在治疗模型中，在关节炎发作后给予ASHE，同时或不给予抗TNF-α抗体。ASHE治疗在CIA发作前显示出疗效，但在CIA形成后没有效果。ASHE治疗延迟了CIA的发作并降低了其严重程度。体外检查表明，ASHE是一种抗氧化剂，并且ASHE抑制人外周血单核细胞中TNF-α和白细胞介素-6的产生。与单独使用抗TNF-α抗体相比，ASHE与抗TNF-α抗体的联合治疗降低了关节炎的严重程度。本研究表明，ASHE对CIA具有预防作用，并支持抗TNF-α抗体的治疗效果。
