Shen Jie, Yang Kai, Jiang Cheng, Ma Xiao-Qiong, Zheng Min-Xia, Sun Cai-Hua
1The First Affiliated Hospital of Zhejiang Chinese Medical University, No. 54 Youdian Road, Hangzhou, 310 006 Zhejiang Province China.
2The First Affiliated Hospital of Medical School of Zhejiang University, Hangzhou, 310 003 Zhejiang Province China.
BMC Chem. 2020 May 2;14(1):35. doi: 10.1186/s13065-020-00687-1. eCollection 2020 Dec.
L. and have been used for hundreds of years to treat hypertension in China. In previous research, there was not a suitable quality control of method for the formulas of L. and It is urgent and essential to develop modern analytical methods for L. and to ensure the quality of the formulas. A rapid approach for simultaneous determination of hyperoside, isoquercitrin and eleutheroside E in L. and by high-performance liquid chromatography with a diode array detector was described and validated. The full method validation, including the linearity, limits of detection and quantification, precision, repeatability, stability and recovery, was examined. All target components, including isomers of hyperoside and isoquercitrin, were baseline separated in 35 min. The developed method was sensitive, reliable and feasible. With this method, the optimal decoction conditions of L. and were selected, and their quality analysis was carried out. Furthermore, an herbal compatibility study of L. and based on detecting variations in the content of their active ingredients was performed by the developed HPLC method. It could be an alternative for the quantitative analysis of herbs that contain hyperoside, isoquercitrin or (and) eleutheroside E in the future.
在中国，淫羊藿和刺五加已被用于治疗高血压数百年。在以往的研究中，对于淫羊藿和刺五加配方没有合适的质量控制方法。开发淫羊藿和刺五加的现代分析方法以确保配方质量迫在眉睫且至关重要。描述并验证了一种采用二极管阵列检测器的高效液相色谱法同时测定淫羊藿和刺五加中金丝桃苷、异槲皮苷和刺五加苷E的快速方法。对包括线性、检测限和定量限、精密度、重复性、稳定性和回收率在内的完整方法验证进行了考察。所有目标成分，包括金丝桃苷和异槲皮苷的异构体，在35分钟内实现基线分离。所建立的方法灵敏、可靠且可行。利用该方法，选择了淫羊藿和刺五加的最佳煎煮条件，并进行了质量分析。此外，通过所建立的高效液相色谱法，基于检测其活性成分含量的变化对淫羊藿和刺五加进行了药对配伍研究。未来它可能成为定量分析含有金丝桃苷、异槲皮苷或（和）刺五加苷E的草药的一种替代方法。
