Martínez-Subiela Silvia, Bernal Luis J, Tvarijonaviciute Asta, Garcia-Martinez Juan D, Tecles Fernando, Cerón Jose J
Vet Dermatol. 2014 Apr;25(2):72-6, e23-4. doi: 10.1111/vde.12108.
Determination of acute phase proteins and markers of oxidative status may be of value for evaluating the severity of disease and the response to treatment. In canine demodicosis, there is no information available regarding the use of such analytes to discriminate between localized and generalized demodicosis or to monitor the response to treatment.
HYPOTHESIS/OBJECTIVES: The aim was to measure analytes related to inflammation and oxidative stress in dogs with localized or generalized demodicosis. In cases of generalized demodicosis, the intention was to study these analytes before and after a period of treatment.
Serum was obtained from three groups: Group 1, healthy dogs; Group 2, dogs with localized demodicosis; and Group 3, dogs with generalized demodicosis.
Animals from Groups 1 and 2 were sampled at the point of diagnosis. Dogs in Group 3 were treated with oral ivermectin 1% at 0.6 mg/kg once daily, and samples were collected at the point of diagnosis and after 30 days of treatment. C-Reactive protein, haptoglobin, albumin, butyrylcholinesterase, paraoxonase-1 and total antioxidant capacity were measured.
Dogs with generalized demodicosis had significantly higher concentrations of C-reactive protein and haptoglobin and lower butyrylcholinesterase activity than dogs in Groups 1 and 2. Dogs in Group 3 also had lower paraoxonase-1 than those in Group 2, The analytes tended to normalize during treatment.
There was an evident acute phase response and changes in selected oxidative state analytes in generalized demodicosis that do not occur in the localized form. These changes could be used for monitoring the response to treatment.
急性期蛋白和氧化状态标志物的测定对于评估疾病严重程度和治疗反应可能具有重要价值。在犬蠕形螨病中，尚无关于使用此类分析物区分局限性和全身性蠕形螨病或监测治疗反应的相关信息。
假设/目的：旨在测量局限性或全身性蠕形螨病犬与炎症和氧化应激相关的分析物。对于全身性蠕形螨病病例，旨在研究治疗一段时间前后的这些分析物。
血清取自三组：第1组，健康犬；第2组，局限性蠕形螨病犬；第3组，全身性蠕形螨病犬。
第1组和第2组动物在诊断时采样。第3组犬每天口服1%伊维菌素，剂量为0.6mg/kg，在诊断时和治疗30天后采集样本。测定C反应蛋白、触珠蛋白、白蛋白、丁酰胆碱酯酶、对氧磷酶-1和总抗氧化能力。
全身性蠕形螨病犬的C反应蛋白和触珠蛋白浓度显著高于第1组和第2组犬，丁酰胆碱酯酶活性则较低。第3组犬的对氧磷酶-1也低于第2组犬，这些分析物在治疗期间趋于正常化。
全身性蠕形螨病存在明显的急性期反应以及所选氧化状态分析物的变化，而局限性蠕形螨病则无此现象。这些变化可用于监测治疗反应。
