Soltani Jalal, Hosseyni Moghaddam Mahdieh S
Phytopathology Department, Bu-Ali Sina University, Hamedan, Iran,
Curr Microbiol. 2014 Sep;69(3):349-56. doi: 10.1007/s00284-014-0594-8. Epub 2014 May 7.
Recent research has shown the bioprospecting of endophytic fungi from Cupressaceae. Here, we further uncover that the healthy cypress plants such as Cupressus arizonica, Cupressus sempervirens var. cereiformis, and Thuja orientalis host highly bioactive endophytic Alternaria fungal species. Indeed, endophytic Alternaria alternata, Alternaria pellucida, and Alternaria tangelonis were recovered from healthy Cupressaceous trees. Biodiversity and bioactivity of recovered endophytic Alternaria species were a matter of biogeography and host identity. We further extracted such Alternaria's metabolites and highlighted their significant antiproliferative, growth inhibitory, and antibacterial activities against the model target fungus Pyricularia oryzae and the model pathogenic bacteria Bacillus sp., Erwinia amylovora, and Pseudomonas syringae. In vitro assays also indicated that endophytic Alternaria species significantly inhibited the growth of cypress fungal phytopathogens Diplodia seriata, Phaeobotryon cupressi, and Spencermartinsia viticola. In conclusion, since the recovered Alternaria species were originally reported as pathogenic and allergenic fungi, our findings suggest a possible ecological niche for them inside the foliar tissues of Cupressaceous trees. Moreover, in this study, the significant bioactivities of endophytic Alternaria species in association with Cupressaceae plant family are reported.
最近的研究表明了对柏科植物内生真菌的生物勘探。在此，我们进一步发现，健康的柏树植物，如亚利桑那柏、柱状柏和侧柏，宿主着具有高度生物活性的链格孢属内生真菌物种。事实上，从健康的柏科树木中分离出了内生链格孢、透明链格孢和柑橘链格孢。所分离出的内生链格孢物种的生物多样性和生物活性是生物地理学和宿主特性的问题。我们进一步提取了此类链格孢的代谢产物，并突出了它们对模式靶标真菌稻瘟病菌以及模式病原菌芽孢杆菌属、梨火疫病菌和丁香假单胞菌具有显著的抗增殖、生长抑制和抗菌活性。体外试验还表明，内生链格孢物种显著抑制了柏树真菌植物病原菌葡萄座腔菌、柏生叶点霉和葡萄生斯氏盘菌的生长。总之，由于所分离出的链格孢物种最初被报道为致病和致敏真菌，我们的研究结果表明它们在柏科树木叶组织内可能具有生态位。此外，在本研究中，还报道了内生链格孢物种与柏科植物家族相关的显著生物活性。
