Laboratory of Cell Culture, UEL/UNOPAR - University Pitágoras Unopar, Research Center in Health Sciences, Av. Paris, 675. Jd. Pizza, Londrina, PR, 86041-140, Brazil.
UNOPAR, Londrina, Brazil.
Lasers Med Sci. 2020 Apr;35(3):523-529. doi: 10.1007/s10103-019-02824-2. Epub 2019 Jun 17.
The aim of the present study was to conduct a systematic review regarding the use of photobiomodulation (PBM) therapy in tumoral cells, addressing the different types of lasers and parameters used. An electronic search was performed in PubMed/MEDLINE, Cochrane Library, and EMBASE databases. The inclusion criteria were being an in vitro study, with tumoral cells, and have at least one group of treatment with low-power laser and a control group (without treatment). Were excluded studies that used only non-carcinogenic cells, in vivo studies, studies that used high-power laser or the association with photosensitizers. The primary outcome evaluated was the cellular viability, and the secondary outcomes were the apoptosis rate and gene and inflammatory mediators' expression. Nineteen studies were identified among the lists of citations of studies that met the exclusion/inclusion criteria. There is a wide divergence regarding the wavelengths and doses used, as well as the type of laser, and each variation leads to different results, with some studies showing an increase in cell proliferation, while other studies showed a decrease. It is possible to suggest that PBM can be used in cancerous lesions in order to decrease the proliferation of these cells depending on the parameters used; however, the lack of standardization of laser irradiation protocols for in vitro investigations does not allow the establishment of ideal parameters for this purpose. Therefore, PBM should be used with caution in cancer patients until more studies are performed.
本研究的目的是对光生物调节（PBM）疗法在肿瘤细胞中的应用进行系统评价，探讨不同类型的激光和使用参数。在 PubMed/MEDLINE、Cochrane 图书馆和 EMBASE 数据库中进行了电子检索。纳入标准为体外研究，使用肿瘤细胞，并且至少有一组低功率激光治疗和一组对照组（无治疗）。排除仅使用非致癌细胞、体内研究、使用大功率激光或与光敏剂联合使用的研究。主要结局评估是细胞活力，次要结局是细胞凋亡率以及基因和炎症介质的表达。在符合排除/纳入标准的研究列表的引文列表中，共确定了 19 项研究。在使用的波长和剂量以及激光类型方面存在广泛的差异，每种变化都会导致不同的结果，一些研究表明细胞增殖增加，而其他研究表明细胞增殖减少。可以认为 PBM 可用于癌症病变，以根据使用的参数减少这些细胞的增殖；然而，由于缺乏体外研究的激光照射方案标准化，因此无法为此目的建立理想的参数。因此，在进行更多研究之前，PBM 在癌症患者中应谨慎使用。
