Firmin-Gomes-Teixeira Z, Anthonioz P
Laboratoire d'Histologie, Faculté de Médecine, Tours.
Biol Struct Morphog. 1989;2(2):45-53.
Immature female Wistar rats were treated with 1 mg of estradiol benzoate for 6 days. The injections were started on the 20th day of age; the animals were autopsied every 3 days after the last injection until the age of 45 days. Islets of hyperplastic cells and metaplasia area were seen in the endocervix in the majority of the animals autopsied. We have the expression of cytokeratin polypeptides in reserve cells, in areas exhibiting reserve cell hyperplasia and squamous metaplasia, using a panel of monoclonal cytokeratin antibodies. The reserve cells were positive for antibodies directed against stratified squamous epithelia, type cytokeratins No. 5, 13 and 17. In addition, hyperplastic cells revealed the presence of cytokeratins No. 7, 8, 18 and 19, specific for simple epithelia, but in a variable manner. The Squamous metaplasia cells exhibited cytokeratins No. 13, 18 and 19, but only weakly reactive. Our observations indicate that estrogen-induced endocervix metaplasia results from a transformation of reserve cells towards an epidermoid type epithelium. Hyperplasia would be the intermediate step in the mechanism of induced cervical metaplasia. This transformation is accompanied by the loss of cytoplasmic keratin proteins and the acquisition of new high molecular weight keratin proteins, specific for stratified squamous epithelia. The basal or reserve cells of the cervix can proliferate to produce regions of squamous cell metaplasia. It appears to be a direct effect of estrogen stimulation. Immunohistochemical staining for different molecular weight keratin proteins may be helpful in the evaluation of reserve cell differentiation.
未成熟雌性Wistar大鼠用1毫克苯甲酸雌二醇处理6天。注射从20日龄开始；在最后一次注射后每3天对动物进行尸检，直至45日龄。在大多数尸检的动物子宫颈中可见增生细胞胰岛和化生区域。我们使用一组单克隆细胞角蛋白抗体，在储备细胞、表现出储备细胞增生和鳞状化生的区域检测到细胞角蛋白多肽的表达。储备细胞对针对复层鳞状上皮的抗体呈阳性，即细胞角蛋白5、13和17型。此外，增生细胞显示存在细胞角蛋白7、8、18和19，这些是单层上皮特有的，但表达情况不一。鳞状化生细胞显示有细胞角蛋白13、18和19，但反应较弱。我们的观察结果表明，雌激素诱导的子宫颈化生是由储备细胞向表皮样上皮类型转化所致。增生可能是诱导性宫颈化生机制中的中间步骤。这种转化伴随着细胞质角蛋白的丢失以及获得复层鳞状上皮特有的新的高分子量角蛋白。子宫颈的基底细胞或储备细胞可增殖产生鳞状细胞化生区域。这似乎是雌激素刺激的直接作用。对不同分子量角蛋白进行免疫组织化学染色可能有助于评估储备细胞的分化。
