Taokaew Siriporn, Nunkaew Natthawut, Siripong Pongpun, Phisalaphong Muenduen
a Chemical Engineering Research Unit for Value Adding of Bioresources, Faculty of Engineering, Department of Chemical Engineering , Chulalongkorn University , Bangkok 10330 , Thailand.
J Biomater Sci Polym Ed. 2014;25(9):907-22. doi: 10.1080/09205063.2014.913464. Epub 2014 May 6.
Bacterial cellulose (BC) films containing an ethanolic extract of mangosteen peel were prepared and their physical, chemical, and anticancer properties were characterized. The cumulative absorption and release profiles of bioactive compounds in the films were determined based on total phenolic and α-mangostin content. The BC films were filled with total phenolic compounds expressed as gallic acid equivalent varying from 4.72 to 275.91 mg/cm3 dried film, and α-mangostin varying from 2.06 to 248.20 mg/cm3 dried film. A Fourier transform infrared spectroscopy evaluation showed that there were weak interactions between the functional groups of the extract and the BC. Decreases in the water absorption capacity and water vapor transmission rate of the modified films were detected. Release studies were performed using Franz diffusion cells. In a non-transdermal system, the release of bioactive compounds from the films depended on concentration, immersion time, and the pH of the dissolution medium. A transdermal diffusion study showed that 59-62% of total phenolic compounds that were initially loaded were released from the films and more than 95% of bioactive compounds released from the films were adsorbed into pig skin. Only very small amount of the bioactive compounds penetrated through pig skin and into phosphate and acetate buffers. In studies of anticancer abilities, the release of 2.0 μg/ml α-mangostin from the BC films could suppress the growth of B16F10 melanoma (approximately 31% survival). With the release of α-mangostin at greater than 17.4-18.4 μg/ml, less than 15 and 5% survival of B16F10 melanoma and MCF-7 breast cancer cells, respectively, was observed.
制备了含有山竹果皮乙醇提取物的细菌纤维素（BC）薄膜，并对其物理、化学和抗癌特性进行了表征。基于总酚和α-倒捻子素含量测定了薄膜中生物活性化合物的累积吸收和释放曲线。BC薄膜中填充的总酚化合物以没食子酸当量表示，范围为4.72至275.91mg/cm³干膜，α-倒捻子素范围为2.06至248.20mg/cm³干膜。傅里叶变换红外光谱评估表明，提取物的官能团与BC之间存在弱相互作用。检测到改性薄膜的吸水能力和水蒸气透过率降低。使用Franz扩散池进行释放研究。在非透皮系统中，薄膜中生物活性化合物的释放取决于浓度、浸泡时间和溶解介质的pH值。透皮扩散研究表明，最初负载的总酚化合物中有59 - 62%从薄膜中释放出来，从薄膜中释放的生物活性化合物中有95%以上被猪皮吸附。只有极少量的生物活性化合物穿透猪皮进入磷酸盐和醋酸盐缓冲液。在抗癌能力研究中，BC薄膜释放2.0μg/ml的α-倒捻子素可抑制B16F10黑色素瘤的生长（存活率约为31%）。当α-倒捻子素释放量大于17.4 - 18.4μg/ml时，观察到B16F10黑色素瘤和MCF - 7乳腺癌细胞的存活率分别低于15%和5%。
