Chemical Engineering Research Unit for Value Adding of Bioresources, Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand; Department of Materials Science and Technology, School of Engineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan.
Chemical Engineering Research Unit for Value Adding of Bioresources, Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand.
Mater Sci Eng C Mater Biol Appl. 2021 Jan;120:111783. doi: 10.1016/j.msec.2020.111783. Epub 2020 Dec 10.
Natural polymeric nanofibers-based materials for medical application is an intensive research area due to the unique features of natural polymeric nanofibers. Bacterial nanocellulose (BC) films containing various concentrations of mangosteen (Garcinia mangostana) peel extract were prepared and evaluated as a multifunctional nanofiber film. The extract was absorbed into BC hydrogel and air dried to entrap the extract into nanofiber network. The resulting films contained about 3, 35, and 294 mg of total phenolic compounds and 2, 24, and 250 mg of α-mangostin per cm of the dried films. The film containing the highest phenolic compounds and α-mangostin performed the inhibitory effect to Staphylococcus epidermidis, Propionibacterium acnes, and Staphylococcus aureus. High anticancer activity against B16F10 melanoma and MCF-7 breast cancer cells having viabilities of 10 and 5%, respectively after 48 h were detected after the treatments with the film. However, the film had a low toxicity against normal fibroblast and keratinocyte cells with 41 and 99% viability, respectively. The research suggested that the prepared films were a multifunctional nanofiber films with antimicrobial and anticancer properties.
基于天然高分子纳米纤维的材料在医学应用中是一个研究热点，这是由于天然高分子纳米纤维具有独特的性质。制备了含有不同浓度山竹（ Garcinia mangostana ）果皮提取物的细菌纳米纤维素（ BC ）薄膜，并将其评估为多功能纳米纤维膜。提取物被吸收到 BC 水凝胶中并风干，以使提取物被捕获到纳米纤维网络中。所得薄膜每平方厘米干燥膜中含有约 3 、 35 和 294mg 的总酚化合物和 2 、 24 和 250mg 的 α-倒捻子素。含有最高酚类化合物和 α-倒捻子素的薄膜对表皮葡萄球菌、痤疮丙酸杆菌和金黄色葡萄球菌表现出抑制作用。经薄膜处理 48 小时后，对 B16F10 黑色素瘤和 MCF-7 乳腺癌细胞的抗癌活性分别检测到 10%和 5%的存活率。然而，该薄膜对正常成纤维细胞和角质形成细胞的毒性较低，其存活率分别为 41%和 99%。研究表明，所制备的薄膜是具有抗菌和抗癌性能的多功能纳米纤维膜。
