Key Laboratory for Advanced Materials & Institute of Fine Chemicals, East China University of Science and Technology , Shanghai, P. R. China.
Anal Chem. 2014 Jun 3;86(11):5502-7. doi: 10.1021/ac501463u. Epub 2014 May 19.
Here, we describe a novel "switch-on" biosensor based on quinonyl glycosides functionalized quantum dots (QDs) for the specific targeting and imaging of transmembrane glycoprotein receptors on the surface of cancer cells. The design of the quinonyl glycosides lies in that the quinone moiety serves as a quencher of QDs and the glycoside moiety as a biospecific ligand for targeting a receptor. We observed that the quenched photoluminescence of the quinone glycosides functionalized QDs could be significantly recovered by a specific lectin that selectively binds to the glycosides clustering the QDs but was not affected by a panel of nonspecific lectins. Moreover, we determined that quinonyl galactoside functionalized QDs could optically image the asialoglycoprotein receptors of a hepatoma cell line in a target-specific manner. This system might provide new insights into the fabrication of photoluminogenic biosensors for the analysis of the universal ligand-receptor recognitions in nature.
在这里，我们描述了一种基于醌基糖苷功能化量子点（QDs）的新型“开启”生物传感器，用于特异性靶向和成像癌细胞表面的跨膜糖蛋白受体。醌基糖苷的设计在于，醌部分作为 QDs 的猝灭剂，而糖苷部分作为生物特异性配体用于靶向受体。我们观察到，通过选择性结合糖苷聚集 QDs 的特定凝集素，可以显著恢复被醌基糖苷功能化 QDs 猝灭的光致发光，而不受一组非特异性凝集素的影响。此外，我们确定醌基半乳糖苷功能化 QDs 可以以靶向特异性的方式光学成像肝癌细胞系的糖蛋白受体。该系统可能为光致发光生物传感器的制造提供新的见解，用于分析自然界中普遍的配体-受体识别。
