He Xiao-Peng, Zhu Bi-Wen, Zang Yi, Li Jia, Chen Guo-Rong, Tian He, Long Yi-Tao
Key Laboratory for Advanced Materials & Institute of Fine Chemicals , East China University of Science and Technology , 130 Meilong Rd. , Shanghai 200237 , PR China . Email:
National Center for Drug Screening , State Key Laboratory of Drug Research , Shanghai Institute of Materia Medica , Chinese Academy of Sciences , Shanghai 201203 , PR China . Email:
Chem Sci. 2015 Mar 1;6(3):1996-2001. doi: 10.1039/c4sc03614j. Epub 2015 Jan 13.
Expression of specific transmembrane receptors by cells frequently represents an important signature of diseases, but this dynamic event can hardly be monitored directly with live cells due to the limitation of current biochemical techniques. Here we develop a pyrenyl glycoanthraquinone construct that can be firmly immobilized on a graphene-spotted screen printed electrode strong π-interactions. The inherent current signal produced by the surface-confined glycoquinone can be used to detect selective sugar-protein recognitions with simple electrochemical techniques and portable facilities. Importantly, we demonstrate that the level of pathogenic receptors expressed by different types of live cells can be tracked with the electrode system in a label-free manner, providing a useful tool for the on-demand disease diagnosis as well as basic biochemical studies.
细胞对特定跨膜受体的表达常常是疾病的一个重要特征，但由于当前生化技术的限制，这一动态过程很难在活细胞中直接监测。在此，我们开发了一种芘基糖蒽醌构建体，它可以通过强π相互作用牢固地固定在石墨烯修饰的丝网印刷电极上。表面受限的糖醌产生的固有电流信号可通过简单的电化学技术和便携式设备用于检测选择性糖蛋白识别。重要的是，我们证明了不同类型活细胞表达的致病受体水平可以通过电极系统以无标记的方式进行追踪，为按需疾病诊断以及基础生化研究提供了一个有用的工具。
