Swyer T W, Strom J, Larson D F
Sarver Heart Center, College of Medicine, The University of Arizona, Tucson, AZ, USA.
Sarver Heart Center, College of Medicine, The University of Arizona, Tucson, AZ, USA
Perfusion. 2014 Nov;29(6):539-43. doi: 10.1177/0267659114534290. Epub 2014 May 6.
Currently there are no medications that can be administered to help deliver more oxygen to the myocardial region experiencing abnormal perfusion. The purpose of this study was to look at the nanoparticle dodecafluoropentane in an emulsion as an oxygen carrier. Using nanoparticles as an oxygen carrier is advantageous because they are able to carry oxygen past blockages that are obstructing red blood cells (6-8 µm) due to their smaller size (250 nm). With the reintroduction of oxygen to the ischemic muscle tissue, a reduced infarct size should be seen.
Male C57BL/6J mice underwent left anterior descending artery (LAD) ligation using 8-0 monofilament nylon suture. Immediately after ligation of the LAD, the control group received a 200-µl intravenous injection of phosphate buffered saline (PBS). The treated group received a dose of 0.6 ml/kg of dodecafluoropentane diluted to a total volume of 200 µl in PBS. The mice were then allowed to recover from anesthesia and were sacrificed 24 hours after the time of ligation. After the mice were sacrificed, the heart was excised and placed at -20°C for 20 minutes. The heart was then sliced into 1-mm sections and stained with tetrazolium red to identify the infarcted area. The area of infarct and ventricle were then analyzed using ImageJ software.
The average area of infarct in comparison to the ventricle for the control mice was 29.3±0.04% compared to 11.7±0.02% for the dodecafluoropentane-treated mice.
The use of dodecafluoropentane in this murine model of myocardial infarction showed a 60% reduction in infarct size (p<0.01). The possibility of using nanoparticles to deliver oxygen to hypoxic tissues has interesting implications and justifies further study.
目前尚无药物可用于帮助向经历异常灌注的心肌区域输送更多氧气。本研究的目的是研究乳液中的纳米颗粒十二氟戊烷作为氧载体的情况。使用纳米颗粒作为氧载体具有优势，因为它们能够携带氧气通过因尺寸较小（250纳米）而阻碍红细胞（6 - 8微米）的堵塞物。随着向缺血肌肉组织重新引入氧气，梗死面积应会减小。
雄性C57BL/6J小鼠使用8 - 0单丝尼龙缝线进行左冠状动脉前降支（LAD）结扎。LAD结扎后立即，对照组接受200微升磷酸盐缓冲盐水（PBS）静脉注射。治疗组接受剂量为0.6毫升/千克的十二氟戊烷，在PBS中稀释至总体积200微升。然后让小鼠从麻醉中恢复，并在结扎后24小时处死。小鼠处死后，取出心脏并置于-20°C下20分钟。然后将心脏切成1毫米厚的切片，用四氮唑红染色以识别梗死区域。然后使用ImageJ软件分析梗死面积和心室面积。
与心室相比，对照小鼠的平均梗死面积为29.3±0.04%，而十二氟戊烷治疗的小鼠为11.7±0.02%。
在这种小鼠心肌梗死模型中使用十二氟戊烷显示梗死面积减少了60%（p<0.01）。使用纳米颗粒向缺氧组织输送氧气的可能性具有有趣的意义，值得进一步研究。
