从开伯尔-普赫图赫瓦省科哈特地区人群皮肤利什曼病临床样本中分离出热带利什曼原虫的聚合酶链反应及显微镜鉴定
PCR and microscopic identification of isolated Leishmania tropica from clinical samples of cutaneous leishmaniasis in human population of Kohat region in Khyber Pakhtunkhwa.
作者信息
Abd El-Salam Nasser M, Ayaz Sultan, Ullah Riaz
机构信息
Arriyadh Community College, King Saud University, P.O. Box 28095, Riyadh 11437, Saudi Arabia.
Department of Zoology, Kohat University of Science and Technology, Kohat 26000, Pakistan.
出版信息
Biomed Res Int. 2014;2014:861831. doi: 10.1155/2014/861831. Epub 2014 Apr 1.
Leishmania tropica was isolated from the clinical patients of cutaneous leishmaniasis in rural community of Kohat district in Khyber Pakhtunkhwa province and was identified through PCR, microscopy, and culture techniques. A total of 113 samples from the clinical patients were examined through PCR, microscopy, and culture which showed 87.61% (99/113), 53.98% (61/113), and 46.90% (53/113) prevalence. During the study, 186 bp Leishmania tropica was identified through PCR. Thus the sensitivity of PCR is very high as compared to the conventional techniques.
热带利什曼原虫是从开伯尔-普赫图赫瓦省科哈特地区农村社区皮肤利什曼病临床患者中分离出来的，并通过聚合酶链反应（PCR）、显微镜检查和培养技术进行鉴定。对113例临床患者的样本进行了PCR、显微镜检查和培养，患病率分别为87.61%（99/113）、53.98%（61/113）和46.90%（53/113）。在研究过程中，通过PCR鉴定出了186bp的热带利什曼原虫。因此，与传统技术相比，PCR的灵敏度非常高。
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