Geng Yiting, Wang Hui, Lu Changqing, Li Qing, Xu Bin, Jiang Jingting, Wu Changping
Department of Oncology, The Third Affiliated Hospital of Soochow University, 185 Juqian Street, Changzhou, 213003, Jiangsu, People's Republic of China.
Int J Clin Oncol. 2015 Apr;20(2):273-81. doi: 10.1007/s10147-014-0701-7. Epub 2014 May 9.
Immune escape plays an important role in tumor progression. In the present study, the expression of B7-H1, B7-H4 and Foxp3 involved in immune escape in gastric carcinoma was investigated and the corresponding clinical significance was evaluated.
Immunohistochemistry was used to detect the expression of B7-H1, B7-H4 and Foxp3 in 100 gastric cancer specimens, and 30 paracarcinoma tissues were used as the control.
Both B7-H1 and B7-H4 showed high expression levels in gastric cancer tissues (65.0 and 71.0 %, respectively), and the expressions of B7-H1 and B7-H4 were positively correlated with the depth of tumor invasion, lymph node metastasis and American Joint Committee on Cancer (AJCC) stage (P < 0.05). The number of Foxp3(+) Tregs was much higher in gastric cancer tissues than control tissues, which was positively correlated with lymph node metastasis (P < 0.05). Similarly, a positive correlation between B7-H1 or B7-H4 expression and the number of Foxp3(+) Tregs was observed. The median overall survival rate of patients with high expression of B7-H1, B7-H4 and Foxp3 was significantly poorer than that of patients with low expression of these proteins (P < 0.05). Cox regression multivariate analysis confirmed that lymph node metastasis, AJCC stage, and B7-H1 and Foxp3 overexpression were independent prognostic factors.
B7-H1, B7-H4 and Foxp3 were overexpressed in gastric cancer tissues. B7-H1 and Foxp3 are negative prognostic factors for patients with gastric cancer.
免疫逃逸在肿瘤进展中起重要作用。本研究探讨了胃癌中参与免疫逃逸的B7-H1、B7-H4和Foxp3的表达情况，并评估了其相应的临床意义。
采用免疫组织化学法检测100例胃癌标本中B7-H1、B7-H4和Foxp3的表达，以30例癌旁组织作为对照。
B7-H1和B7-H4在胃癌组织中均呈高表达（分别为65.0%和71.0%），且B7-H1和B7-H4的表达与肿瘤浸润深度、淋巴结转移及美国癌症联合委员会（AJCC）分期呈正相关（P<0.05）。胃癌组织中Foxp3(+)调节性T细胞数量明显高于对照组织，且与淋巴结转移呈正相关（P<0.05）。同样，观察到B7-H1或B7-H4表达与Foxp3(+)调节性T细胞数量呈正相关。B7-H1、B7-H4和Foxp3高表达患者的中位总生存率明显低于这些蛋白低表达患者（P<0.05）。Cox回归多因素分析证实，淋巴结转移、AJCC分期以及B7-H1和Foxp3过表达是独立的预后因素。
B7-H1、B7-H4和Foxp3在胃癌组织中过表达。B7-H1和Foxp3是胃癌患者的不良预后因素。
