Ramautar Rawi, de Jong Gerhardus J
Analytical Biosciences, LACDR, Leiden University, PO Box 9502, 2300 RA, Leiden, The Netherlands.
Bioanalysis. 2014 Apr;6(7):1011-26. doi: 10.4155/bio.14.51.
Metabolomics is the comprehensive analysis of low molecular weight compounds in biological samples such as cells, body fluids and tissues. Comprehensive profiling of metabolites in complex sample matrices with the current analytical toolbox remains a huge challenge. Over the past few years, liquid chromatography-mass spectrometry (LC-MS) and capillary electrophoresis-mass spectrometry (CE-MS) have emerged as powerful complementary analytical techniques in the field of metabolomics. This Review provides an update of the most recent developments in LC-MS and CE-MS for metabolomics. Concerning LC-MS, attention is paid to developments in column technology and miniaturized systems, while strategies are discussed to improve the reproducibility and the concentration sensitivity of CE-MS for metabolomics studies. Novel interfacing techniques for coupling CE to MS are also considered. Representative examples illustrate the potential of the recent developments in LC-MS and CE-MS for metabolomics. Finally, some conclusions and perspectives are provided.
代谢组学是对生物样品（如细胞、体液和组织）中低分子量化合物进行的全面分析。利用当前的分析工具对复杂样品基质中的代谢物进行全面分析仍然是一项巨大的挑战。在过去几年中，液相色谱 - 质谱联用（LC-MS）和毛细管电泳 - 质谱联用（CE-MS）已成为代谢组学领域强大的互补分析技术。本综述提供了用于代谢组学的LC-MS和CE-MS最新进展的更新。关于LC-MS，重点关注柱技术和小型化系统的发展，同时讨论了提高CE-MS在代谢组学研究中的重现性和浓度灵敏度的策略。还考虑了用于CE与MS联用的新型接口技术。代表性的例子说明了LC-MS和CE-MS在代谢组学方面最新进展的潜力。最后，给出了一些结论和展望。
