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在 20°C 的膜生物反应器中从城市污水污泥中富集反硝化甲烷营养菌。

Enrichment of denitrifying methanotrophic bacteria from municipal wastewater sludge in a membrane bioreactor at 20°C.

机构信息

Sub-department of Environmental Technology, Wageningen University, P.O. Box 17, 6700 AA, Wageningen, The Netherlands.

Sub-department of Environmental Technology, Wageningen University, P.O. Box 17, 6700 AA, Wageningen, The Netherlands.

出版信息

J Hazard Mater. 2014 Jun 15;274:428-35. doi: 10.1016/j.jhazmat.2014.04.031. Epub 2014 Apr 23.

DOI:10.1016/j.jhazmat.2014.04.031
PMID:24809733
Abstract

Simultaneous nitrogen and methane removal by the slow growing denitrifying methanotrophic bacterium 'Candidatus Methylomirabilis oxyfera' offers opportunities for a new approach to wastewater treatment. However, volumetric nitrite consumption rates should be increased by an order of magnitude before application in wastewater treatment becomes possible. A maximum volumetric nitrite consumption rate of 36 mg NO2(-)-N/L d was achieved in a membrane bioreactor inoculated with wastewater sludge and operated at 20°C. This rate is similar to maximum rates reported in literature, though it was thought that by strict biomass retention using membranes, higher rates would be achieved. In experiments lasting several years, growth was not stable: every experiment showed a decrease in activity after 1-2 years. The cause remains unknown. Rates increased after addition of copper and operating a membrane bioreactor at shorter hydraulic retention times. Further research should focus on long-term effects of copper addition and operation at hydraulic retention times in the order of hours using membrane bioreactors.

摘要

通过生长缓慢的反硝化甲烷营养菌“Candidatus Methylomirabilis oxyfera”同时去除氮和甲烷为废水处理提供了一种新的方法。然而,在将其应用于废水处理之前,其亚硝酸盐的比消耗速率应该提高一个数量级。在接种废水污泥并在 20°C 下运行的膜生物反应器中,达到了 36 mg NO2(-)-N/L/d 的最大比亚硝酸盐消耗速率。该速率与文献中报道的最大速率相似,尽管人们认为通过使用膜严格保留生物质,将实现更高的速率。在持续数年的实验中,生长并不稳定:每个实验在 1-2 年后都显示出活性下降。原因尚不清楚。在添加铜并将膜生物反应器在更短的水力停留时间下运行后,速率增加。进一步的研究应集中在使用膜生物反应器在几小时的水力停留时间下添加铜和操作的长期影响上。

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