Sub-department of Environmental Technology, Wageningen University, Wageningen, The Netherlands.
J Hazard Mater. 2012 Aug 15;227-228:164-71. doi: 10.1016/j.jhazmat.2012.05.032. Epub 2012 May 15.
Despite many advantages of anaerobic sewage treatment over conventional activated sludge treatment, it has not yet been applied in temperate zones. This is especially because effluent from low-temperature anaerobic treatment contains nitrogen and dissolved methane. The presence of nitrogen and methane offers the opportunity to develop a reactor in which methane is used as electron donor for denitrification. Such a reactor could be used in a new concept for low-temperature anaerobic sewage treatment, consisting of a UASB-digester system, a reactor for denitrification coupled to anaerobic methane oxidation, and a nitritation reactor. In the present study denitrifying methanotrophic bacteria similar to 'Candidatus Methylomirabilis oxyfera' were enriched. Maximum volumetric nitrite consumption rates were 33.5 mg NO(2)(-)-N/Ld (using synthetic medium) and 37.8 mg NO(2)(-)-N/Ld (using medium containing effluent from a sewage treatment plant), which are similar to the maximum rate reported so far. Though the goal was to increase the rates, in both reactors, after reaching these maximum rates, volumetric nitrite consumption rates decreased in time. Results indicate biomass washout may have significantly decelerated enrichment. Therefore, to obtain higher volumetric consumption rates, further research should focus on systems with complete biomass retention.
尽管厌氧污水处理相对于传统的活性污泥处理具有许多优势，但它在温带地区尚未得到应用。这主要是因为低温厌氧处理的出水含有氮和溶解的甲烷。氮和甲烷的存在为开发一种以甲烷作为反硝化电子供体的反应器提供了机会。这种反应器可以用于一种新型的低温厌氧污水处理概念，该概念包括 UASB 消化器系统、与厌氧甲烷氧化耦合的反硝化反应器和亚硝化反应器。本研究中富集了类似于“产氧甲烷八叠球菌”的反硝化甲烷营养菌。最大的亚硝酸氮消耗速率为 33.5 mg NO2--N/Ld（使用合成培养基）和 37.8 mg NO2--N/Ld（使用含有污水处理厂出水的培养基），与迄今为止报道的最大速率相似。尽管目标是提高速率，但在两个反应器中，达到这些最大速率后，亚硝酸氮的消耗速率随时间下降。结果表明，生物量洗出可能显著减缓了富集。因此，为了获得更高的体积消耗速率，进一步的研究应集中在具有完全生物质保留的系统上。
