Mamalis A, Fiadorchanka N, Adams L, Serravallo M, Heilman E, Siegel D, Brody N, Jagdeo J
Department of Dermatology, UC Davis, Sacramento, California, USA.
Dermatology Service, Sacramento VA Medical Center, Mather, California, USA.
J Drugs Dermatol. 2014 May;13(5):574-578.
Ultraviolet (UV) radiation results in a significant loss in years of healthy life, approximately 1.5 million disability-adjusted life years (DALYs), and is associated with greater than 60,000 deaths annually worldwide that are attributed to melanoma and other skin cancers. Currently, there are no standardized biomarkers or assay panels to assess oxidative stress skin injury patterns in human skin exposed to ionizing radiation. Using biopsy specimens from chronic solar UV-exposed and UV-protected skin, we demonstrate that UV radiation-induced oxidative skin injury can be evaluated by an immunohistochemical panel that stains 8-hydroxydeoxyguanosine (8-OH-dG) to assess DNA adducts, 4-hydroxy-2-nonenal (HNE) to assess lipid peroxidation, and advanced glycation end products (AGEs) to assess protein damage. We believe this panel contains the necessary cellular biomarkers to evaluate topical agents, such as sunscreens and anti-oxidants that are designed to prevent oxidative skin damage and may reduce UV-associated skin aging, carcinogenesis, and inflammatory skin diseases. We envision that this panel will become an important tool for researchers developing topical agents to protect against UV radiation and other oxidants and ultimately lead to reductions in lost years of healthy life, DALYs, and annual deaths associated with UV radiation.
紫外线（UV）辐射导致健康生命年显著损失，约150万个伤残调整生命年（DALYs），并且在全球每年与超过60000例归因于黑色素瘤和其他皮肤癌的死亡相关。目前，尚无标准化的生物标志物或检测组来评估暴露于电离辐射的人体皮肤中的氧化应激皮肤损伤模式。利用来自长期日光紫外线照射皮肤和紫外线防护皮肤的活检标本，我们证明紫外线辐射诱导的氧化皮肤损伤可通过一个免疫组织化学检测组进行评估，该检测组对8-羟基脱氧鸟苷（8-OH-dG）进行染色以评估DNA加合物，对4-羟基-2-壬烯醛（HNE）进行染色以评估脂质过氧化，对晚期糖基化终产物（AGEs）进行染色以评估蛋白质损伤。我们认为该检测组包含评估局部用药（如旨在预防氧化皮肤损伤并可能减少紫外线相关皮肤老化、致癌作用和炎症性皮肤病的防晒霜和抗氧化剂）所需的细胞生物标志物。我们设想该检测组将成为研究人员开发预防紫外线辐射和其他氧化剂的局部用药的重要工具，并最终减少与紫外线辐射相关的健康生命年损失、伤残调整生命年和年度死亡人数。
