Park Jae Jun, Chon Nu Ri, Lee Young Ju, Park Hyojin
Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea.
J Neurogastroenterol Motil. 2015 Jul 30;21(3):352-60. doi: 10.5056/jnm14112.
BACKGROUND/AIMS: There are limited therapeutic options available for irritable bowel syndrome with diarrhea (IBS-D). We tested the effects of Atractylodes japonica rhizome, a perennial plant native to North Asia, on both upper and lower gastrointestinal (GI) motility in guinea pigs.
The extract of A. japonica rhizome was administered orally at different doses to test its effects on upper GI motility as determined from charcoal transit in native guinea pigs and in guinea pigs pretreated with thyrotropin-releasing hormone or mustard oil. Regarding its effect on lower GI motility, the removed guinea pig colon was suspended in a chamber containing Krebs-Henseleit solution and the transit time of artificial feces was measured with various dilutions of the extract. As for in vivo assay, weight and number of fecal pellets expelled were determined under the same drug preparation used in upper GI motility experiment.
The extract of A. japonica rhizome had no significant effect on upper GI motility in either normal or altered physiological states. However, the extract increased colonic transit time in the in vitro model. In the fecal expulsion study, the cumulative weight and number of pellets did not differ significantly between the control group and groups treated with the extracts. In the animals pretreated in vivo with thyrotropin-releasing hormone, however, the weight and number of fecal pellets were sig-nificantly decreased in animals treated with 300 mg/kg and 600 mg/kg doses of extract.
Our findings suggest that the extract of A. japonica rhizome can be a potential agent for IBS-D.
背景/目的：腹泻型肠易激综合征（IBS-D）的治疗选择有限。我们测试了北亚多年生植物白术根茎对豚鼠上、下胃肠道（GI）动力的影响。
对豚鼠口服不同剂量的白术根茎提取物，通过观察正常豚鼠以及用促甲状腺激素释放激素或芥子油预处理的豚鼠中木炭的传输情况，来测试其对上胃肠道动力的影响。关于其对下胃肠道动力的影响，将去除的豚鼠结肠悬挂在含有克雷布斯-亨泽莱特溶液的腔室中，并用不同稀释度的提取物测量人工粪便的传输时间。至于体内试验，在与上胃肠道动力实验相同的药物制剂条件下，测定排出粪便颗粒的重量和数量。
白术根茎提取物在正常或改变的生理状态下对上胃肠道动力均无显著影响。然而，该提取物在体外模型中增加了结肠传输时间。在粪便排出研究中，对照组与提取物处理组之间粪便颗粒的累积重量和数量没有显著差异。然而，在体内用促甲状腺激素释放激素预处理的动物中，用300mg/kg和600mg/kg剂量提取物处理的动物粪便颗粒的重量和数量显著减少。
我们的研究结果表明，白术根茎提取物可能是一种治疗IBS-D的潜在药物。
