关于血管紧张素II与血管紧张素II互补RNA编码的肽在溶液中可能相互作用的核磁共振研究。
NMR study of the possible interaction in solution of angiotensin II with a peptide encoded by angiotensin II complementary RNA.
作者信息
Eaton H L, Austin R E, Fesik S W, Martin S F
机构信息
Pharmaceutical Discovery Division, Abbott Laboratories, Abbott Park, IL 60064.
出版信息
Proc Natl Acad Sci U S A. 1989 Dec;86(24):9767-9. doi: 10.1073/pnas.86.24.9767.
The potential binding of angiotensin II (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe) (AII) to a peptide encoded by its complementary RNA (Lys-Gly-Val-Asp-Val-Tyr-Ala-Val) (IIA) has been studied by monitoring the 1H NMR spectrum of IIA in aqueous phosphate or Tris.HCl buffer (2H2O) as it is titrated with AII. For molar ratios of AII/IIA ranging from 0.2 to 1.8, the NMR spectra are unchanged as compared to the spectra of the isolated peptides. Based on these findings, the Kd for the putative biomolecular complex of the two peptides under these conditions is calculated to be greater than 10(-4) M. This result does not support the suggestion of Elton et al. [Elton, T. S., Dion, L.D., Bost, K. L., Oparil, S. & Blalock, J. E. (1988) Proc. Natl. Acad. Sci. USA 85, 2518-2522] that AII and IIA engage in high-affinity binding (Kd approximately 5 x 10(-8) M) with each other.
通过监测在磷酸盐水溶液或Tris.HCl缓冲液（2H₂O）中用血管紧张素II（Asp-Arg-Val-Tyr-Ile-His-Pro-Phe）（AII）滴定其互补RNA编码的肽（Lys-Gly-Val-Asp-Val-Tyr-Ala-Val）（IIA）时IIA的¹H NMR谱，研究了AII与IIA的潜在结合。对于AII/IIA的摩尔比范围为0.2至1.8，与分离肽的谱相比，NMR谱没有变化。基于这些发现，计算出在这些条件下两种肽的假定生物分子复合物的Kd大于10⁻⁴ M。该结果不支持Elton等人[Elton, T. S., Dion, L.D., Bost, K. L., Oparil, S. & Blalock, J. E. (1988) Proc. Natl. Acad. Sci. USA 85, 2518-2522]提出的AII和IIA彼此进行高亲和力结合（Kd约为5×10⁻⁸ M）的建议。
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