Goodarzi M, Moosavi-Movahedi A A, Habibi-Rezaei M, Shourian M, Ghourchian H, Ahmad F, Farhadi M, Saboury A A, Sheibani N
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran; Center of Excellence in Biothermodynamics, University of Tehran, Tehran, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2014 Sep 15;130:561-7. doi: 10.1016/j.saa.2014.04.056. Epub 2014 Apr 20.
Protein glycation is a cascade of nonenzymatic reactions between reducing sugars and amino groups of proteins. It is referred to as fructation when the reducing monosaccharide is fructose. Some potential mechanisms have been suggested for the generation of reactive oxygen species (ROS) by protein glycation reactions in the presence of glucose. In this state, glucose autoxidation, ketoamine, and oxidative advance glycation end products (AGEs) formation are considered as major sources of ROS and perhaps heme degradation during hemoglobin glycation. However, whether fructose mediated glycation produces ROS and heme degradation is unknown. Here we report that ROS (H2O2) production occurred during hemoglobin fructation in vitro using chemiluminescence methods. The enhanced heme exposure and degradation were determined using UV-Vis and fluorescence spectrophotometry. Following accumulation of ROS, heme degradation products were accumulated reaching a plateau along with the detected ROS. Thus, fructose may make a significant contribution to the production of ROS, glycation of proteins, and heme degradation during diabetes.
蛋白质糖基化是还原糖与蛋白质氨基之间的一系列非酶促反应。当还原单糖为果糖时，它被称为果糖化。对于在葡萄糖存在下蛋白质糖基化反应产生活性氧（ROS）的现象，已经提出了一些潜在机制。在这种状态下，葡萄糖自氧化、酮胺以及氧化晚期糖基化终产物（AGEs）的形成被认为是ROS的主要来源，并且在血红蛋白糖基化过程中可能也是血红素降解的主要来源。然而，果糖介导的糖基化是否产生活性氧和血红素降解尚不清楚。在此我们报告，使用化学发光方法在体外血红蛋白果糖化过程中发生了ROS（H2O2）的产生。使用紫外可见光谱和荧光分光光度法测定了血红素暴露和降解的增强情况。随着ROS的积累，血红素降解产物也随之积累，并随着检测到的ROS达到一个平台期。因此，果糖可能在糖尿病期间ROS的产生、蛋白质糖基化和血红素降解中起重要作用。
