Institute of Biochemistry & Biophysics, University of Tehran, Tehran, Iran.
Department of Cell & Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Colloids Surf B Biointerfaces. 2019 May 1;177:188-195. doi: 10.1016/j.colsurfb.2019.01.046. Epub 2019 Jan 23.
Nowadays diabetes, as a metabolic disorder, is increasing at an alarming rate. Glycation and production of advanced glycation end products (AGEs) is the most important factor involved in diabetic complications. Due to the side effects of synthetic drugs, the demand for natural anti-diabetic herbal medicines has increased. Propolis is a natural and resinous material, which iscollected by honeybees. Due to the impact of nanotechnology in medicine and the advantageous role of nanoparticles in treatment, nano-propolis particles (PNP) were prepared. The anti-glycation effect of PNP at various concentrations was investigated on human hemoglobin (Hb) glycation and fructation and compared with aspirin as a common anti-glycation agent using glycation specific AGE fluorescence, AGE-specific absorbance and circular dichroism (CD) methods. Fluorescence spectroscopy results showed that PNP inhibited the formation of AGEs in Hb glycation and fructation by glucose and fructose, respectively. CD results revealed that PNP caused an increase in Hb beta-sheet content while decreasing the alpha helical content. Additionally, the results of UV-Vis spectroscopy and fluorescence emission of heme degradation products revealed the protective effect of PNP on heme during glycation and fructation of human Hb. It is notable that the synergistic effects of combined propolis nanoparticles and aspirin is more than either of them alone. However, having said that, PNP as a natural product has a potential to be an effective drug in the treatment of diabetes.
如今，糖尿病作为一种代谢紊乱，其发病率正以惊人的速度增长。糖基化和晚期糖基化终产物（AGEs）的产生是糖尿病并发症的最重要因素。由于合成药物的副作用，对天然抗糖尿病草药的需求增加了。蜂胶是一种天然的树脂状物质，由蜜蜂采集。由于纳米技术在医学中的应用以及纳米粒子在治疗中的优势作用，制备了纳米蜂胶颗粒（PNP）。研究了 PNP 在不同浓度下对人血红蛋白（Hb）糖化和果糖化的抗糖基化作用，并与阿司匹林作为常见的抗糖基化剂进行了比较，采用糖基化特定的 AGE 荧光、AGE 特异性吸光度和圆二色性（CD）方法。荧光光谱结果表明，PNP 抑制了葡萄糖和果糖分别在 Hb 糖化和果糖化过程中 AGEs 的形成。CD 结果表明，PNP 导致 Hbβ-折叠含量增加，同时α螺旋含量减少。此外，UV-Vis 光谱和血红素降解产物的荧光发射结果表明，PNP 在人 Hb 糖化和果糖化过程中对血红素有保护作用。值得注意的是，蜂胶纳米粒子和阿司匹林联合使用的协同效应大于它们各自单独使用的效果。话虽如此，PNP 作为一种天然产物，具有成为治疗糖尿病的有效药物的潜力。
