Azeredo Joana, Sillankorva Sanna, Pires Diana P
Centre of Biological Engineering, University of Minho, Campus de Gualtar, Braga, Portugal,
Methods Mol Biol. 2014;1149:23-32. doi: 10.1007/978-1-4939-0473-0_4.
Bacterial viruses or bacteriophages were discovered nearly 100 years ago and are ubiquitous in nature, readily isolated for a variety of bacterial hosts and from a diversity of sources. Here, we describe the methods used to isolate, concentrate, purify, and produce bacteriophages specific for Pseudomonas species.
细菌病毒或噬菌体在近100年前就被发现了,它们在自然界中无处不在,很容易从各种细菌宿主和不同来源中分离出来。在这里,我们描述了用于分离、浓缩、纯化和生产针对假单胞菌属细菌的噬菌体的方法。