Panigrahi P, Mohanty S B
Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park.
Arch Virol. 1989;109(1-2):125-33. doi: 10.1007/BF01310524.
A defective transport of bovine parainfluenza-3 virus (PI-3V) hemagglutinin-neuraminidase (HN) glycoprotein was evidenced in interferon (IFN)-treated bovine turbinate (BTu) cells. Indirect immunofluorescence performed with monoclonal antibody to PI-3 HN glycoprotein demonstrated accumulation of this protein in the perinuclear cytoplasm of IFN-treated cells. Untreated, infected control cells had a generalized widespread fluorescence. Unfixed control cells showed a uniform surface fluorescence in contrast to a few specs of fluorescence on the plasma membrane of IFN-treated cells. Electron microscopic localization of HN protein was done by immuno-gold ultrastructural cytochemistry. Untreated cells had uniform gold label on the plasma membrane and around the budding virus particles with no label in the cytoplasm. In IFN-treated cells, however, there was an accumulation of gold particles in the cytoplasm with only a few particles on the cell surface. Quantitative analysis of HN protein on the cell surface by solid phase radioimmune-assay revealed a greater amount of this protein on the surface of control cells, than those on the IFN-treated cells.
在经干扰素（IFN）处理的牛鼻甲（BTu）细胞中，证实存在牛副流感3型病毒（PI - 3V）血凝素神经氨酸酶（HN）糖蛋白的转运缺陷。用针对PI - 3 HN糖蛋白的单克隆抗体进行间接免疫荧光检测显示，该蛋白在经IFN处理的细胞的核周细胞质中积累。未经处理的感染对照细胞呈现广泛的荧光。未固定的对照细胞显示出均匀的表面荧光，与之形成对比的是，经IFN处理的细胞的质膜上只有少量荧光斑点。通过免疫金超微结构细胞化学方法对HN蛋白进行电子显微镜定位。未经处理的细胞在质膜和出芽病毒颗粒周围有均匀的金标记，细胞质中无标记。然而，在经IFN处理的细胞中，细胞质中有金颗粒积累，细胞表面只有少量颗粒。通过固相放射免疫测定对细胞表面的HN蛋白进行定量分析，结果显示对照细胞表面的该蛋白量比经IFN处理的细胞更多。
