一种用于荧光活细胞成像的新型全水溶性铜（I）探针。
A novel fully water-soluble Cu(I) probe for fluorescence live cell imaging.
作者信息
Giuffrida Maria Laura, Rizzarelli Enrico, Tomaselli Gaetano A, Satriano Cristina, Trusso Sfrazzetto Giuseppe
机构信息
Institute of Biostructure and Bioimaging, National Research Council, Viale Andrea Doria, Catania 95125, Italy.
出版信息
Chem Commun (Camb). 2014 Sep 7;50(69):9835-8. doi: 10.1039/c4cc02147a.
A new Cu(I) probe (OBEP-CS1), bearing an alkyl-pyridinium moiety to get complete water solubility, was synthesized. Fluorescence titrations support copper binding and high selectivity in a water solution whereas formation of aggregates was excluded by DOSY experiments. The detection capability for intracellular Cu(I), especially localized in mitochondria, was demonstrated in differentiated neuroblastoma cells by confocal microscopy.
合成了一种新的Cu(I)探针（OBEP-CS1），其带有烷基吡啶鎓部分以实现完全水溶性。荧光滴定法证明了在水溶液中铜的结合和高选择性，而扩散排序光谱实验排除了聚集体的形成。通过共聚焦显微镜在分化的神经母细胞瘤细胞中证明了对细胞内Cu(I)（特别是定位于线粒体中的）的检测能力。
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