Périamé M, Pagès J-M, Davin-Regli A
UMR-MD-1, Aix-Marseille Université, IRBA, Transporteurs Membranaires, Chimiorésistance et Drug Design, Marseille, France.
Int J Cosmet Sci. 2014 Aug;36(4):386-95. doi: 10.1111/ics.12140. Epub 2014 Jul 1.
The aim of this study was to obtain a better understanding regarding the origin of recurrent contamination by Enterobacter gergoviae in diverse cosmetic formula. We studied 65 isolates collected from various sources (clinical, food, cosmetics).
RAPD analysis using AP12H, REP and ERIC-PCR was carried out for epidemiological typing. Evaluation of susceptibility to preservatives currently used in cosmetics for a representative panel of collection strains was measured. Preservative efficacy was evaluated by minimum inhibitory concentrations and minimum bactericidal concentrations (MBCs).
Eighty per cent of isolates was unrelated. E. gergoviae showed significant levels of resistance to preservatives. MBC was higher than maximum permitted concentrations imposed by European Commission (EC). Association of preservatives showed in rare case additive effects, and no synergic effects were observed.
Most of the cosmetic formulations are contaminated with unrelated E. gergoviae strains. Maximum allowed concentrations for sodium benzoate are inefficient to limit proliferation and control adaptability to this bacterium in cosmetic products. Efflux mechanisms should be involved in methylisothiazolinone-chloromethylisothiazolinone and triclosan adaptation.
本研究旨在更好地了解不同化妆品配方中格氏肠杆菌反复污染的来源。我们研究了从各种来源（临床、食品、化妆品）收集的65株分离株。
使用AP12H、REP和ERIC-PCR进行RAPD分析以进行流行病学分型。对一组代表性的收集菌株测定了对化妆品中目前使用的防腐剂的敏感性。通过最低抑菌浓度和最低杀菌浓度（MBC）评估防腐剂效力。
80%的分离株不相关。格氏肠杆菌对防腐剂表现出显著的抗性水平。MBC高于欧盟委员会规定的最大允许浓度。防腐剂联合使用在极少数情况下显示出相加作用，未观察到协同作用。
大多数化妆品配方被不相关的格氏肠杆菌菌株污染。苯甲酸钠的最大允许浓度不足以限制该细菌在化妆品中的增殖和控制其适应性。外排机制可能参与了甲基异噻唑啉酮 - 氯甲基异噻唑啉酮和三氯生的适应性。
