Pedersen-Bjergaard U, Nielsen L B, Jensen K, Edvinsson L, Jansen I, Olesen J
Department of Neurology, Gentofte Hospital, University of Copenhagen, Denmark.
Peptides. 1989 Nov-Dec;10(6):1147-52. doi: 10.1016/0196-9781(89)90006-5.
Neurokinin A (NKA), substance P (SP) and the two peptides combined (SP + NKA) were injected intracutaneously on the forearm and into the temporal muscle of healthy volunteers. Pain intensity, cutaneous wheal and flare responses and tenderness of the temporal muscle were quantitated. SP but not NKA induced cutaneous pain. This relates the algesic effect of SP to the specific N-terminal amino acid sequence of the peptide, not shared by NKA. NKA, however, potentiated the algesic effect of SP as SP + NKA induced a significantly prolonged cutaneous pain sensation. Both peptides induced wheals, but only SP induced flare. These results confirm previous studies relating wheal formation to the identical C-terminal amino acid sequence of the two peptides and flare reaction to the N-terminal part of SP. Injections into the temporal muscle did not cause pain or tenderness.
将神经激肽A（NKA）、P物质（SP）以及两种肽联合使用（SP + NKA），经皮注射到健康志愿者的前臂和颞肌中。对疼痛强度、皮肤风团和潮红反应以及颞肌压痛进行定量分析。SP可诱发皮肤疼痛，而NKA则不能。这表明SP的致痛作用与该肽特定的N端氨基酸序列有关，而NKA不具有此序列。然而，NKA可增强SP的致痛作用，因为SP + NKA可诱发明显延长的皮肤疼痛感觉。两种肽均可诱发风团，但只有SP可诱发潮红。这些结果证实了先前的研究，即风团形成与两种肽相同的C端氨基酸序列有关，而潮红反应与SP的N端部分有关。注射到颞肌中不会引起疼痛或压痛。
