Eom Jun Sik, Lee Shin Ja, Kim Hyun Sang, Choi Youyoung, Jo Seong Uk, Lee Sang Suk, Kim Eun Tae, Lee Sung Sill
Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea.
University Centered Labs, Gyeongsang National University, Jinju 52828, Korea.
J Anim Sci Technol. 2022 Mar;64(2):247-261. doi: 10.5187/jast.2022.e14. Epub 2022 Mar 31.
Ketosis is associated with high milk yield during lactating or insufficient feed intake in lactating dairy cows. However, few studies have been conducted on the metabolomics of ketosis in Korean lactating dairy cows. The present study aimed to investigate the serum and urine metabolites profiling of lactating dairy cows through proton nuclear magnetic resonance (H-NMR) spectroscopy and comparing those between healthy (CON) and subclinical ketosis (SCK) groups. Six lactating dairy cows were categorized into CON and SCK groups. All experimental Holstein cows were fed total mixed ration. Serum and urine samples were collected from the jugular vein of the neck and by hand sweeping the perineum, respectively. The metabolites in the serum and urine were determined using H-NMR spectroscopy. Identification and quantification of metabolites was performed by Chenomx NMR Suite 8.4 software. Metabolites statistical analysis was performed by Metaboanalyst version 5.0 program. In the serum, the acetoacetate level was significantly ( < 0.05) higher in the SCK group than in the CON group, and whereas acetate, galactose and pyruvate levels tended to be higher. CON group had significantly ( < 0.05) higher levels of 5-aminolevulinate and betaine. Indole-3-acetate, theophylline, p-cresol, 3-hydroxymandelate, gentisate, -acetylglucosamine, -nitrosodimethylamine, xanthine and pyridoxine levels were significantly ( < 0.05) higher in the urine of the SCK group than that in the CON group, which had higher levels of homogentisate, ribose, gluconate, ethylene glycol, maltose, 3-methyl-2-oxovalerate and glycocholate. Some significantly ( < 0.05) different metabolites in the serum and urine were associated with ketosis diseases, inflammation, energy balance and body weight. This study will be contributed useful a future ketosis metabolomics studies in Korea.
酮病与泌乳期奶牛产奶量高或采食量不足有关。然而，关于韩国泌乳奶牛酮病代谢组学的研究很少。本研究旨在通过质子核磁共振（H-NMR）光谱研究泌乳奶牛的血清和尿液代谢物谱，并比较健康（CON）组和亚临床酮病（SCK）组之间的差异。将六头泌乳奶牛分为CON组和SCK组。所有实验用荷斯坦奶牛均饲喂全混合日粮。分别从颈部颈静脉和通过手动擦拭会阴收集血清和尿液样本。使用H-NMR光谱测定血清和尿液中的代谢物。代谢物的鉴定和定量由Chenomx NMR Suite 8.4软件进行。代谢物统计分析由Metaboanalyst 5.0版程序进行。在血清中，SCK组的乙酰乙酸水平显著（<0.05）高于CON组，而乙酸盐、半乳糖和丙酮酸水平有升高趋势。CON组的5-氨基乙酰丙酸和甜菜碱水平显著（<0.05）较高。SCK组尿液中吲哚-3-乙酸、茶碱、对甲酚、3-羟基扁桃酸、龙胆酸盐、N-乙酰葡糖胺、N-亚硝基二甲胺、黄嘌呤和吡哆醇水平显著（<0.05）高于CON组，而CON组的尿黑酸、核糖、葡萄糖酸盐、乙二醇、麦芽糖、3-甲基-2-氧代戊酸和甘氨胆酸盐水平较高。血清和尿液中一些显著（<0.05）不同的代谢物与酮病、炎症、能量平衡和体重有关。本研究将为韩国未来的酮病代谢组学研究提供有用的参考。
