Wang Shanshan, Nie Yao, Xu Yan, Zhang Rongzhen, Ko Tzu-Ping, Huang Chun-Hsiang, Chan Hsiu-Chien, Guo Rey-Ting, Xiao Rong
School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.
Chem Commun (Camb). 2014 Jul 25;50(58):7770-2. doi: 10.1039/c4cc01752h.
Structure-guided design of substrate-binding pocket inversed the stereoselectivity of an NADH-dependent medium-chain alcohol dehydrogenase (MDR) from Prelog to anti-Prelog. The pocket-forming amino acids, especially the unconserved residues as hotspots, play critical roles in directing MDRs' stereoselectivity.
基于结构的底物结合口袋设计逆转了依赖烟酰胺腺嘌呤二核苷酸（NADH）的中链醇脱氢酶（MDR）的立体选择性，使其从普雷洛格型转变为反普雷洛格型。形成口袋的氨基酸，尤其是作为热点的非保守残基，在决定MDR的立体选择性方面起着关键作用。
