Institute of Marine Biology, National Taiwan Ocean University, No. 2, Pei-Ning Road, Keelung 20224, Taiwan, ROC.
Center of Excellence for the Oceans, National Taiwan Ocean University, No. 2, Pei-Ning Road, Keelung 20224, Taiwan, ROC.
J Gen Virol. 2014 Aug;95(Pt 8):1799-1808. doi: 10.1099/vir.0.064782-0. Epub 2014 May 16.
White spot syndrome virus (WSSV) is a large enveloped virus which has caused severe mortality and huge economic losses in the shrimp farming industry. The enveloped virus must be combined with the receptors of the host cell membrane by the virus envelope proteins. In the case of WSSV, binding of envelope proteins with receptors of the host cell membrane was discovered in a number of previous studies, such as VP53A and 10 other proteins with chitin-binding protein (CBP), VP28 with Penaeus monodon Rab7, VP187 with β-integrin, and so on. WSSV envelope proteins were also considered capable of forming a protein complex dubbed an 'infectome'. In this study, the research was focused on the role of CBP in the WSSV infection process, and the relationship between CBP and the envelope proteins VP24, VP28, VP31, VP32 VP39B, VP53A and VP56. The results of the reverse transcription-PCR analyses showed that CBP existed in a variety of shrimp. The speed of WSSV infection could be slowed down by inhibiting CBP gene expression. Far-Western blot analysis and His pull-down assays were conducted, and a protein complex was found that appeared to be composed of a 'linker' protein consisting of VP31, VP32 and VP39B together with four envelope proteins, including VP24, VP28, VP53A and VP56. This protein complex was possibly another part of the infectome and the possible binding region with CBP. The findings of this study may have identified certain points for further WSSV research.
白斑综合征病毒（WSSV）是一种大型包膜病毒，它给虾养殖业造成了严重的死亡率和巨大的经济损失。包膜病毒必须通过病毒包膜蛋白与宿主细胞膜上的受体结合。在 WSSV 的情况下，之前的一些研究发现包膜蛋白与宿主细胞膜上的受体结合，如 VP53A 和其他 10 种具有几丁质结合蛋白（CBP）的蛋白、VP28 与斑节对虾 Rab7、VP187 与β-整合素等。WSSV 包膜蛋白也被认为能够形成一个被称为“感染组”的蛋白质复合物。在这项研究中，研究的重点是 CBP 在 WSSV 感染过程中的作用，以及 CBP 与包膜蛋白 VP24、VP28、VP31、VP32、VP39B、VP53A 和 VP56 之间的关系。逆转录-PCR 分析的结果表明，CBP 存在于各种虾中。通过抑制 CBP 基因表达，可以减缓 WSSV 的感染速度。进行了 Far-Western blot 分析和 His 下拉测定，发现了一个似乎由 VP31、VP32 和 VP39B 组成的“连接”蛋白与包括 VP24、VP28、VP53A 和 VP56 在内的四种包膜蛋白组成的蛋白质复合物。这个蛋白质复合物可能是感染组的另一个部分，也是与 CBP 可能的结合区域。本研究的结果可能为进一步研究 WSSV 提供了某些要点。
