Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China.
State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China.
Int J Mol Sci. 2020 Feb 13;21(4):1243. doi: 10.3390/ijms21041243.
Kuruma prawn, , has the third largest annual yield among shrimp species with vital economic significance in China. White spot syndrome virus (WSSV) is a great threat to the global shrimp farming industry and results in high mortality. Pellino, a highly conserved E3 ubiquitin ligase, has been found to be an important modulator of the Toll-like receptor (TLR) signaling pathways that participate in the innate immune response and ubiquitination. In the present study, the Pellino gene from was identified. A qRT-PCR assay showed the presence of MjPellino in all the tested tissues and revealed that the transcript level of this gene was significantly upregulated in both the gills and hemocytes after challenge with WSSV and . The function of MjPellino was further verified at the protein level. The results of the three-dimensional modeling and protein-protein docking analyses and a GST pull-down assay revealed that the MjPellino protein was able to bind to the WSSV envelope protein VP26. In addition, the knockdown of MjPellino in vivo significantly decreased the expression of MjAMPs. These results suggest that MjPellino might play an important role in the immune response of kuruma prawn.
日本囊对虾，是中国产量第三大的对虾品种，具有重要的经济意义。白斑综合征病毒（WSSV）是全球对虾养殖业的巨大威胁，可导致高死亡率。Pellino 是一种高度保守的 E3 泛素连接酶，已被发现是参与先天免疫反应和泛素化的 Toll 样受体（TLR）信号通路的重要调节剂。本研究从 中鉴定出了 Pellino 基因。qRT-PCR 检测表明，该基因在所有检测组织中均存在，并且在受到 WSSV 和 刺激后，该基因在鳃和血细胞中的转录水平显著上调。在蛋白质水平进一步验证了 MjPellino 的功能。三维建模和蛋白-蛋白对接分析以及 GST 下拉实验的结果表明，MjPellino 蛋白能够与 WSSV 囊膜蛋白 VP26 结合。此外，体内敲低 MjPellino 显著降低了 MjAMPs 的表达。这些结果表明，MjPellino 可能在日本囊对虾的免疫反应中发挥重要作用。
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