新型光可裂解连接子：α-硫代苯乙酮型连接子。

New photocleavable linker: α-thioacetophenone-type linker.

作者信息

Yonezawa Hirotaka, Nishiyama Yoshitake, Takeo Koji, Iwatsubo Takeshi, Tomita Taisuke, Yokoshima Satoshi, Fukuyama Tohru

机构信息

Graduate School of Pharmaceutical Sciences, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Graduate School of Pharmaceutical Sciences, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Graduate School of Pharmaceutical Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.

出版信息

Bioorg Med Chem Lett. 2014 Jul 1;24(13):2831-3. doi: 10.1016/j.bmcl.2014.04.104. Epub 2014 May 9.

DOI:10.1016/j.bmcl.2014.04.104

PMID:24841630

Abstract

Photocleavable linkers are advantageous over the common linkers because they could be cleaved without using reagents. A novel photocleavable linker with an α-thioacetophenone moiety has been developed. This linker, which can be cleaved upon irradiation at 365 nm via the Norrish type II reaction, is applicable to a protein affinity purification system, allowing target proteins to be effectively isolated. This novel linker would serve as an effective tool in chemical biology.

摘要

光可裂解连接子比普通连接子更具优势，因为它们无需使用试剂即可裂解。一种带有α-硫代苯乙酮部分的新型光可裂解连接子已被开发出来。这种连接子在365nm光照下可通过Norrish II型反应裂解，适用于蛋白质亲和纯化系统，能有效分离目标蛋白质。这种新型连接子将成为化学生物学中的一种有效工具。