Kim In Sik, Yang Eun Ju, Shin Dong-Ha, Son Kwang-Hee, Park Ho-Yong, Lee Ji-Sook
Department of Biomedical Laboratory Science, School of Medicine, Eulji University, Daejeon 301‑746, Republic of Korea.
Department of Clinical Laboratory Science, College of Health and Therapy, Daegu Hanny University, Gyeongsan 712‑715, Republic of Korea.
Mol Med Rep. 2014 Aug;10(2):1025-9. doi: 10.3892/mmr.2014.2231. Epub 2014 May 13.
Arazyme is a novel extracellular metalloprotease secreted by Aranicola proteolyticus. Endothelial cells are involved in the pathogenesis of a number of inflammatory diseases, induce uncontrolled cell viability and express various inflammatory mediators, including cytokines, chemokines, adhesion molecules and reactive oxygen species (ROS). In the current study, human umbilical vein endothelilal cells (HUVECs) were used to investigate the anti‑inflammatory effects of arazyme following lipopolysaccharide (LPS) stimulation. Apoptosis of HUVECs due to LPS was inhibited by arazyme. In various inflammatory responses induced by LPS, arazyme inhibited the secretion of the monocyte chemoattractant protein‑1 and interleukin‑6, and the expression of vascular cell adhesion molecule‑1 and intercellular adhesion molecule‑1. Arazyme also suppressed ROS production in HUVECs. The action of arazyme was not associated with NF‑κB activity in HUVECs. These results indicate that arazyme has anti‑inflammatory properties in inflamed endothelial cells and may be useful as a therapeutic agent for inflammatory diseases associated with endothelial cells.
Arazyme是一种由解蛋白阿拉尼科拉菌分泌的新型细胞外金属蛋白酶。内皮细胞参与多种炎症性疾病的发病机制，可导致细胞活力失控，并表达多种炎症介质，包括细胞因子、趋化因子、黏附分子和活性氧（ROS）。在本研究中，使用人脐静脉内皮细胞（HUVECs）来研究Arazyme在脂多糖（LPS）刺激后产生的抗炎作用。Arazyme可抑制因LPS导致的HUVECs凋亡。在LPS诱导的各种炎症反应中，Arazyme可抑制单核细胞趋化蛋白-1和白细胞介素-6的分泌，以及血管细胞黏附分子-1和细胞间黏附分子-1的表达。Arazyme还可抑制HUVECs中ROS的产生。Arazyme的作用与HUVECs中的NF-κB活性无关。这些结果表明，Arazyme在炎症内皮细胞中具有抗炎特性，可能作为一种治疗与内皮细胞相关炎症性疾病的药物。
