Seigneurin D, Cohen O, Louis J
Quantitative Cytology Group, University Joseph Fourier, Grenoble, France.
Anal Cell Pathol. 1989 Apr;1(2):97-104.
Breast cancer imprints from 93 patients were assayed for the presence of progesterone receptors (PgR) using a monoclonal antibody (Transbio) and an immunocytochemical assay (ICA) method which stains only the epithelial cell nuclei. Results were compared with conventional biochemical PgR determinations (DCCA) and were in qualitative agreement in 86% of the cases. Quantitative analyses were done on PgR-ICA- and Feulgen-stained imprints from 32 tumours using a SAMBA 2005 cell image processor. Results obtained showed a high correlation between DCCA values and the P product derived from the mean PgR concentration of marked tumour cells and the percentage of marked cells. Intra-tumoral and intra-cell heterogeneity were featured and showed relation to tumour differentiation and size.
使用单克隆抗体（Transbio）和仅对上皮细胞核进行染色的免疫细胞化学分析（ICA）方法，对93例患者的乳腺癌印记进行孕酮受体（PgR）检测。将结果与传统生化PgR测定法（DCCA）进行比较，86%的病例在定性上一致。使用SAMBA 2005细胞图像处理器对32个肿瘤的PgR-ICA和Feulgen染色印记进行定量分析。所得结果显示，DCCA值与源自标记肿瘤细胞平均PgR浓度和标记细胞百分比的P乘积之间具有高度相关性。肿瘤内和细胞内的异质性较为明显，且与肿瘤分化和大小有关。
