Cornelisse C J, Van Driel-Kulker A M
Cytometry. 1985 Sep;6(5):471-7. doi: 10.1002/cyto.990060512.
A DNA image cytometry method, implemented on the LEYTAS image processing system, has been applied to acriflavine-Feulgen-stained breast cancer cytology specimens. An essential feature of the LEYTAS image cytometry method (LCM) is the automated selection of single nuclei according to predetermined specifications. Visual interaction has been used to reject remaining artefacts like overlapping nuclei. DNA profiles obtained with LCM have been compared with DNA profiles obtained by scanning cytophotometry (SCM) or flow cytometry (FCM). The resolution of DNA profiles obtained with LCM is similar to that from SCM but lower than that from FCM. However, a high correlation is found for the DNA indices measured with LCM and FCM (r = 0.97). The LCM profiles of aneuploid tumours generally showed lower accessory diploid fractions than FCM profiles due to the automated rejection of leukocyte nuclei. Also, LCM profiles frequently showed the presence of minor subpopulations of highly aneuploid/polyploid tumour cells that could not be identified by FCM. Therefore, LCM appears to be supplementary to FCM for studying tumour cell stemline heterogeneity.
一种在LEYTAS图像处理系统上实现的DNA图像细胞术方法已应用于吖啶黄素-福尔根染色的乳腺癌细胞学标本。LEYTAS图像细胞术方法（LCM）的一个基本特征是根据预定规格自动选择单个细胞核。视觉交互已被用于排除诸如重叠细胞核等剩余的假象。用LCM获得的DNA图谱已与通过扫描细胞光度术（SCM）或流式细胞术（FCM）获得的DNA图谱进行了比较。用LCM获得的DNA图谱的分辨率与SCM的相似，但低于FCM的。然而，发现用LCM和FCM测量的DNA指数具有高度相关性（r = 0.97）。由于自动排除了白细胞核，非整倍体肿瘤的LCM图谱通常显示出比FCM图谱更低的附属二倍体分数。此外，LCM图谱经常显示存在高度非整倍体/多倍体肿瘤细胞的次要亚群，而这些亚群无法通过FCM识别。因此，在研究肿瘤细胞系异质性方面，LCM似乎是FCM的补充。
