College of Pharmacy, Yeungnam University, Gyeongsan, 712-749, Korea.
Arch Pharm Res. 2015;38(5):776-84. doi: 10.1007/s12272-014-0402-9. Epub 2014 May 22.
A HPLC-DAD method was developed for simultaneous determination of four marker compounds, kaempferol-3-O-rutinoside, safflomin A, safflomin B and bidenoside C, in the extract of the flowers of Carthamus tinctorius Linne. Natural samples were extracted in 50% aqueous methanol by ultra-sonication for 60 min. Marker compounds were separated on a C18 column by two-step gradient elution of mobile phase (acetonitrile/water) at a flow rate of 0.75 mL/min and detected at 210 nm. The retention times of safflomin A and safflomin B were shifted according to the pH of the mobile phase. The optimized analytical method was validated to confirm linearity, precision, accuracy and stability of marker compounds. The method was successfully employed to analyze 17 natural samples from different regions, and the data matrix was monitored and visualized by principal component analysis. The assay method could be applied for quality control of the flowers of C. tinctorius Linne.
建立了高效液相色谱-二极管阵列检测法（HPLC-DAD）同时测定红花中 4 种指标性成分：山柰酚-3-O-芸香糖苷、山柰酚-3-O-新橙皮糖苷、山柰酚-3-O-槐糖苷和柳穿鱼叶苷 C 的含量。采用超声辅助提取法，以 50%甲醇水溶液为提取溶剂，60 min 内提取完毕。采用梯度洗脱方式，以乙腈-水为流动相，在 C18 色谱柱上进行分离，流速为 0.75 mL/min，检测波长为 210nm。山柰酚-3-O-新橙皮糖苷和山柰酚-3-O-槐糖苷的保留时间随流动相 pH 值的变化而发生位移。对优化后的分析方法进行线性、精密度、准确度和稳定性考察。该方法成功用于分析 17 个不同产地的天然样品，并通过主成分分析对数据矩阵进行监测和可视化。该测定方法可用于红花的质量控制。
