Pankongadisak Porntipa, Ruktanonchai Uracha Rungsardthong, Supaphol Pitt, Suwantong Orawan
School of Science, Mae Fah Luang University, Tasud, Muang, Chiang Rai, 57100, Thailand.
AAPS PharmSciTech. 2014 Oct;15(5):1105-15. doi: 10.1208/s12249-014-0140-9. Epub 2014 May 23.
Silver nanoparticles (AgNPs)-loaded alginate beads embedded in gelatin scaffolds were successfully prepared. The AgNPs-loaded calcium alginate beads were prepared by electrospraying method. The effect of alginate concentration and applied voltage on shape and diameter of beads was studied. The diameter of dry AgNPs-loaded calcium alignate beads at various concentrations of AgNO3 ranged between 154 and 171 μm. The AgNPs-loaded calcium alginate beads embedded in gelatin scaffolds were fabricated by freeze-drying method. The water swelling and weight loss behaviors of the AgNPs-loaded alginate beads embedded in gelatin scaffolds increased with an increase in the submersion time. Moreover, the genipin-cross-linked gelatin scaffolds were proven to be nontoxic to normal human dermal fibroblasts, suggesting their potential uses as wound dressings.
成功制备了嵌入明胶支架中的载银纳米颗粒（AgNPs）海藻酸盐珠。通过电喷雾法制备了载AgNPs的海藻酸钙珠。研究了海藻酸盐浓度和施加电压对珠形状和直径的影响。在不同浓度的AgNO3下，干燥的载AgNPs海藻酸钙珠的直径在154至171μm之间。通过冷冻干燥法制备了嵌入明胶支架中的载AgNPs海藻酸钙珠。嵌入明胶支架中的载AgNPs海藻酸盐珠的水溶胀和失重行为随浸没时间的增加而增加。此外，已证明京尼平交联的明胶支架对正常人皮肤成纤维细胞无毒，表明它们作为伤口敷料的潜在用途。
