Neuner Gilbert, Kuprian Edith
Unit Functional Plant Biology, Stressphysiology and Climate Resistance, Institute of Botany, University of Innsbruck, Sternwartestr. 15, 6020, Innsbruck, Austria,
Methods Mol Biol. 2014;1166:91-8. doi: 10.1007/978-1-4939-0844-8_9.
Infrared thermal analysis is an invaluable technique to study the plant freezing process. In the differential mode infrared thermal analysis allows to localize ice nucleation and ice propagation in whole plants or plant samples at the tissue level. Ice barriers can be visualized, and supercooling of cells, tissues, and organs can be monitored. Places where ice masses are accommodated in the apoplast can be identified. Here, we describe an experimental setting developed in the laboratory in Innsbruck, give detailed information on the practical procedure and preconditions, and give additionally an idea of the problems that can be encountered and how they by special precautions may be overcome.
红外热分析是研究植物冷冻过程的一项极为重要的技术。在差分模式下，红外热分析能够在组织层面定位全株植物或植物样本中的冰核形成与冰的传播。冰屏障可以可视化，细胞、组织和器官的过冷现象也能够被监测到。可以识别质外体中容纳冰块的位置。在此，我们描述了在因斯布鲁克实验室开发的一种实验装置，给出了实际操作步骤和前提条件的详细信息，此外还说明了可能遇到的问题以及如何通过特殊预防措施加以克服。
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