Department of Botany, Unit Functional Plant Biology, Stress Physiology, University of Innsbruck, Innsbruck, Austria.
Methods Mol Biol. 2020;2156:33-41. doi: 10.1007/978-1-0716-0660-5_4.
Infrared thermal analysis is an invaluable technique to study the plant freezing process. In the differential mode, infrared thermal analysis (IDTA) allows to localize ice nucleation and ice propagation in whole plants or plant samples at the tissue level. Ice barriers can be visualized and supercooling of cells, tissues, and organs can be monitored. Places where ice masses are accommodated in the apoplast can be identified. Here, we describe an experimental setting developed in our laboratory, give detailed information on the practical procedure and preconditions, and give additionally an idea of the problems that would be encountered and how they may be overcome.
红外热分析是研究植物冷冻过程的一种非常有价值的技术。在差示模式下，红外热分析（IDTA）可以在组织水平上定位整个植物或植物样本中的冰核形成和冰传播。可以可视化冰障，并监测细胞、组织和器官的过冷。可以识别冰团在质外体中容纳的位置。在这里，我们描述了我们实验室开发的一种实验设置，详细介绍了实际程序和前提条件，并提供了遇到的问题和如何克服这些问题的思路。
