Lukacheva M, Schramek S
Tr Inst Im Pastera. 1989;66:104-9, 172.
Phospholipids are a part of the outside membrane of Coxiella burnetii (or other gram negative bacteria). It is possible to extract them with a mixture of chloroform-methanol (CM). For extraction of phospholipids from C. burnetii it is important to take account of temperature, proportion of CM, and reaction time. The best results are obtained at CM 2:1, at boiling point of this mixture and reaction time of 2 hours. Longer reaction time does not influence the reaction, the additional amounts of phospholipids can be extracted only with fresh solvent mixture.
磷脂是伯氏考克斯氏体（或其他革兰氏阴性菌）外膜的一部分。可以用氯仿 - 甲醇（CM）混合物将它们提取出来。从伯氏考克斯氏体中提取磷脂时，考虑温度、CM的比例和反应时间很重要。在CM为2:1、该混合物的沸点以及2小时的反应时间条件下可获得最佳结果。更长的反应时间不会影响反应，只能用新鲜的溶剂混合物提取额外的磷脂量。
