克隆成熟脂肪细胞在细胞分裂前需要将增殖能力的子细胞从脂肪中输出。
Clonal Mature Adipocyte Production of Proliferative-competent Daughter Cells Requires Lipid Export Prior to Cell Division.
机构信息
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China ; Department of Animal Science, Washington State University, Pullman, WA 99164.
Departamento de Producción Agraria, Universidad Pública de Navarra, Pamplona 31006 Navarra, Spain.
出版信息
Int J Stem Cells. 2009 May;2(1):76-9. doi: 10.15283/ijsc.2009.2.1.76.
BACKGROUND AND OBJECTIVES
Numerous in vitro observations have been published to show that mature adipocytes may resume proliferation and begin to populate the adipofibroblast fraction or form other cell types.
METHODS AND RESULTS
In the present study, we evaluated clonal cultures of mature pig-derived adipocytes as they began to reestablish their ability to divide. The lipid contained within the cytoplasm was either moved to the apical ends of the cell, or large droplets were physically extruded from the cell. In the latter case, we ascertained that the cell lipid droplet was handled in a different manner to that by beef-derived adipocytes as described in other published studies.
CONCLUSIONS
Pig-derived adipocytes expel large amounts of lipid directly into the medium environment prior to becoming capable of cell division, rather than retaining all lipids like the beef cells. This difference in lipid handling and trafficking may be a novel mechanism in adipocyte resumption of proliferation.
背景和目的
已有大量体外观察结果表明，成熟脂肪细胞可能会重新开始增殖，并开始填充脂肪成纤维细胞部分或形成其他细胞类型。
方法和结果
在本研究中，我们评估了成熟猪源脂肪细胞的克隆培养，因为它们开始重新建立分裂的能力。细胞质内的脂质要么转移到细胞的顶端，要么大的液滴从细胞中被挤出。在后一种情况下，我们确定细胞脂滴的处理方式与其他已发表的研究中描述的牛源脂肪细胞不同。
结论
猪源脂肪细胞在能够进行细胞分裂之前，会将大量的脂质直接排出到培养基环境中，而不像牛细胞那样保留所有的脂质。这种脂质处理和运输的差异可能是脂肪细胞重新开始增殖的一个新机制。
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