Gonçalves Cátia, Martins Marta, Diniz Mário S, Costa Maria H, Caeiro Sandra, Costa Pedro M
IMAR - Instituto do Mar, Departamento de Ciências e Engenharia do Ambiente, Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
IMAR - Instituto do Mar, Departamento de Ciências e Engenharia do Ambiente, Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa, 2829-516 Caparica, Portugal; IPMA - Instituto Português do Mar e da Atmosfera, Avenida do Brasil, 1449-006 Lisboa, Portugal.
Mar Environ Res. 2014 Aug;99:170-8. doi: 10.1016/j.marenvres.2014.04.012. Epub 2014 May 9.
Within an environmental risk assessment framework of a moderately contaminated estuary (the Sado, SW Portugal), the present work intended to detect endocrine disruption in a flatfish, Solea senegalensis Kaup, 1858, and its potential relationship to organic toxicants. Animals were collected from two distinct areas in the estuary (industrial and rural) and from an external reference area. Hepatic vitellogenin (VTG) levels, cytochrome P450 (CYP1A) induction, ethoxyresorufin-O-deethylase (EROD) activity plus gonad histology were analysed. Males and females were sexually immature and showed no significant evidence of degenerative pathologies. However, hepatic VTG concentrations in males from the industrial area were higher than Reference, even reaching levels comparable to females, indicating low but measurable oestrogenic effects caused by the complex contaminant mixture in estuarine sediments. These individuals also presented elevated CYP1A induction and EROD activity, which is consistent with contamination by organic toxicants such as PAHs and other aryl hydrocarbon receptor (Ahr) -mediated toxicants.
在一个中等污染河口（葡萄牙西南部的萨多河）的环境风险评估框架内，本研究旨在检测一种比目鱼（塞内加尔鳎，1858年命名）的内分泌干扰情况及其与有机毒物的潜在关系。从河口的两个不同区域（工业区和农村区）以及一个外部参考区域采集了动物样本。分析了肝脏卵黄蛋白原（VTG）水平、细胞色素P450（CYP1A）诱导情况、乙氧基异吩唑酮 - O - 脱乙基酶（EROD）活性以及性腺组织学。雄性和雌性均未达到性成熟，且未显示出明显的退行性病变迹象。然而，工业区雄性个体的肝脏VTG浓度高于参考值，甚至达到了与雌性相当的水平，这表明河口沉积物中的复杂污染物混合物导致了低水平但可测量的雌激素效应。这些个体还表现出CYP1A诱导和EROD活性升高，这与多环芳烃等有机毒物以及其他芳烃受体（Ahr）介导的毒物污染情况一致。
