Martins Carla, Alves de Matos António P, Costa Maria H, Costa Pedro M
MARE - Marine and Environmental Sciences Centre, Departamento de Ciências e Engenharia do Ambiente, Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
Centro de Investigação Interdisciplinar Egas Moniz (CiiEM), Quinta da Granja, Monte de Caparica, 2829-511 Caparica, Portugal.
Mar Environ Res. 2015 Dec;112(Pt A):122-30. doi: 10.1016/j.marenvres.2015.10.007. Epub 2015 Oct 19.
Juvenile Solea senegalensis were exposed in the laboratory (ex situ) and field (in situ) to different sediments of a moderately impacted estuary (the Sado, Portugal) for 28 days. A qualitative histopathological screening yielded scant lesions to gills, albeit alterations such as epithelial hyperplasia being evident and more frequent in fish exposed ex situ. Fully quantitative traits, namely chloride and goblet cell count and size revealed differences between the two bioassay approaches, with ex situ experiments likely enhancing bioavailability of toxicants. Chloride cells endured autolytic processes that could, at least in part, relate to contamination by mixed metals and polycyclic aromatic hydrocarbons (PAHs). Goblet cells did not reveal changes in the chemistry of mucous. Still, their number and size was reduced in fish exposed ex situ to the sediments most contaminated by PAHs, with evidence for adaptation. Also, copper histochemistry revealed the potential role of mucocytes in the regulation of metals.
在实验室（异地）和野外（原位）将塞内加尔鳎幼鱼暴露于一个受到中度影响的河口（葡萄牙萨多河）的不同沉积物中28天。定性组织病理学筛查显示鳃部病变较少，尽管上皮增生等改变很明显，并且在异地暴露的鱼类中更频繁出现。完全定量特征，即氯化物细胞和杯状细胞计数及大小，揭示了两种生物测定方法之间的差异，异地实验可能提高了有毒物质的生物有效性。氯化物细胞经历了自溶过程，这至少部分可能与混合金属和多环芳烃（PAHs）污染有关。杯状细胞未显示黏液化学性质的变化。然而，在异地暴露于受PAHs污染最严重的沉积物的鱼类中，它们的数量和大小减少，有适应的证据。此外，铜组织化学揭示了黏液细胞在金属调节中的潜在作用。
