Yang Zui-Su, Xu Yin-Feng, Huang Fang-Fang, Ding Guo-Fang
Engineering Research Centers of Marine Organism Medical Products, Medical College of Zhejiang Ocean University, Zhoushan 316022, China.
Molecules. 2014 May 23;19(5):6851-62. doi: 10.3390/molecules19056851.
We studied the expression of the non-metastatic clone 23 type 1 (nm23H1) gene, vascular endothelial growth factor (VEGF)-C, and its receptor VEGFR-3 using an in situ hybridization technique and immunohistochemical analyses with prostate cancer tissues and adjacent benign tissues of 52 human archival cases. The association between VEGF-C expression, microlymphatic count (MLC), and staining intensity for nm23H1 and VEGFR-3 was used to evaluate tumor metastasis and survival rate. MLC values were significantly higher in tumorous tissue than in non-cancerous tissue. VEGF-C mRNA, VEGFR-3, and nm23H1 were highly expressed in tumorous tissue. VEGFR-3 expression was greater in VEGF-C mRNA-positive tumors than in VEGF-C mRNA-negative tumors. The association of VEGFR-3 expression with VEGF-C mRNA and MLC suggested that the poor prognosis and tumor metastasis associated with VEGFR-3 expression may be due, in part, to its role in promoting angiogenesis. VEGF-C expression was significantly associated with tumor lymphangiogenesis, angiogenesis, and immune response as a potent multifunctional stimulating factor in prostate cancer. Expression of nm23H1 was significantly inversely correlated with lymph node metastasis. Furthermore, there was a strong negative correlation between the expression of nm23H1, VEGF-C mRNA, and MLC. These findings provide important information for prophylactic, diagnostic, and therapeutic strategies for prostate cancer.
我们采用原位杂交技术以及免疫组织化学分析方法，对52例人类存档病例的前列腺癌组织及相邻良性组织中1型非转移克隆23（nm23H1）基因、血管内皮生长因子（VEGF）-C及其受体VEGFR-3的表达情况进行了研究。通过分析VEGF-C表达、微淋巴管计数（MLC）以及nm23H1和VEGFR-3的染色强度之间的关联，来评估肿瘤转移和生存率。肿瘤组织中的MLC值显著高于非癌组织。VEGF-C mRNA、VEGFR-3和nm23H1在肿瘤组织中高表达。VEGFR-3在VEGF-C mRNA阳性肿瘤中的表达高于VEGF-C mRNA阴性肿瘤。VEGFR-3表达与VEGF-C mRNA和MLC的关联表明，与VEGFR-3表达相关的预后不良和肿瘤转移可能部分归因于其在促进血管生成中的作用。VEGF-C表达作为前列腺癌中一种强大的多功能刺激因子，与肿瘤淋巴管生成、血管生成和免疫反应显著相关。nm23H1的表达与淋巴结转移显著负相关。此外，nm23H1、VEGF-C mRNA的表达与MLC之间存在强烈的负相关。这些发现为前列腺癌的预防、诊断和治疗策略提供了重要信息。
