• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

VX2荷瘤兔腹膜后淋巴结中肿瘤诱导的VEGF-C过表达

Tumor-induced VEGF-C overexpression in retroperitoneal lymph nodes in VX2 carcinoma-bearing rabbits.

作者信息

Huang Yong-Wen, Zhou Yun, Lan Chun-Yan, Wang Yin, Feng Yan-Ling, Luo Rong-Zhen, Liu Ji-Hong

机构信息

Department of Gynecology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, People's Republic of China.

Department of Pathology, Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou, People's Republic of China.

出版信息

Drug Des Devel Ther. 2015 Nov 5;9:5949-56. doi: 10.2147/DDDT.S89810. eCollection 2015.

DOI:10.2147/DDDT.S89810
PMID:26604693
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4639523/
Abstract

OBJECTIVE

To establish the retroperitoneal lymph node (RLN) metastasis model of cervical carcinoma in rabbits and evaluate the relationship of vascular endothelial growth factor-C (VEGF-C) expression and the lymph node status.

METHODS

Forty-eight rabbits were injected with VX2 cells or RPMI solution at muscular mucosae of the myometrium 0.5 cm away from the cervix. Animals were treated with or without cis-diamminedichloroplatinum(II) (cisplatin: DDP) and sacrificed on days 15, 21, and 27 post-VX2 or RPMI injections. Tumor mass and RLNs were examined histopathologically. Quantitative real-time PCR was used to examine the changes in VEGF-C mRNA expression. Levels of VEGF-C protein expression in tissues were determined using immunohistochemistry staining.

RESULTS

Development of VX2 cervical carcinoma and the RLNs metastasis was confirmed with pathological examination. Significantly increased tumor volume was observed on days 15, 21, and 27 postinjection (P<0.05). The enlargement of RLNs was found on day 21. Expression of VEGF-C was significantly upregulated in peripheral white blood cells, tumor mass, and RLNs in an association with cancer progression. DDP resulted in a suppression of VEGF-C expression, whereas the influences on tumor mass and lymphatic metastasis were insignificant.

CONCLUSION

Elevated VEGF-C expressions in peripheral white blood cells and RLNs are associated with tumor progression and lymphatic metastasis. DDP treatment inhibits VEGF-C expression and fails to protect against metastatic cervical cancer.

摘要

目的

建立兔宫颈癌腹膜后淋巴结转移模型，评估血管内皮生长因子C（VEGF-C）表达与淋巴结状态的关系。

方法

48只兔在距宫颈0.5 cm的子宫肌层肌黏膜处注射VX2细胞或RPMI溶液。对动物进行或不进行顺二氯二氨铂（顺铂：DDP）治疗，并在注射VX2或RPMI后第15、21和27天处死。对肿瘤块和腹膜后淋巴结进行组织病理学检查。采用定量实时PCR检测VEGF-C mRNA表达的变化。用免疫组织化学染色法测定组织中VEGF-C蛋白表达水平。

结果

病理检查证实VX2宫颈癌及腹膜后淋巴结转移形成。注射后第15、21和27天观察到肿瘤体积显著增大（P<0.05）。第21天发现腹膜后淋巴结肿大。VEGF-C在外周白细胞、肿瘤块和腹膜后淋巴结中的表达随癌症进展而显著上调。DDP导致VEGF-C表达受到抑制，但其对肿瘤块和淋巴转移的影响不显著。

结论

外周白细胞和腹膜后淋巴结中VEGF-C表达升高与肿瘤进展和淋巴转移有关。DDP治疗可抑制VEGF-C表达，但不能预防转移性宫颈癌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb0/4639523/d6e002c9048a/dddt-9-5949Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb0/4639523/6904fa8416ba/dddt-9-5949Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb0/4639523/a60fb199cc54/dddt-9-5949Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb0/4639523/16dfefa0b6f3/dddt-9-5949Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb0/4639523/6112b8e9c302/dddt-9-5949Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb0/4639523/d6e002c9048a/dddt-9-5949Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb0/4639523/6904fa8416ba/dddt-9-5949Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb0/4639523/a60fb199cc54/dddt-9-5949Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb0/4639523/16dfefa0b6f3/dddt-9-5949Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb0/4639523/6112b8e9c302/dddt-9-5949Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cb0/4639523/d6e002c9048a/dddt-9-5949Fig5.jpg

相似文献

1
Tumor-induced VEGF-C overexpression in retroperitoneal lymph nodes in VX2 carcinoma-bearing rabbits.VX2荷瘤兔腹膜后淋巴结中肿瘤诱导的VEGF-C过表达
Drug Des Devel Ther. 2015 Nov 5;9:5949-56. doi: 10.2147/DDDT.S89810. eCollection 2015.
2
VEGF-c expression in an in vivo model of orthotopic endometrial cancer and retroperitoneal lymph node metastasis.VEGF-c在原位子宫内膜癌及腹膜后淋巴结转移体内模型中的表达
Reprod Biol Endocrinol. 2013 May 21;11:49. doi: 10.1186/1477-7827-11-49.
3
Semaphorin 4D expression is associated with a poor clinical outcome in cervical cancer patients.信号素4D的表达与宫颈癌患者不良的临床预后相关。
Microvasc Res. 2014 May;93:1-8. doi: 10.1016/j.mvr.2014.02.007. Epub 2014 Mar 3.
4
Establishment of the retroperitoneal lymph node metastasis model of endometrial VX2 carcinoma in rabbits and observation of its metastatic features.兔子宫内膜VX2癌腹膜后淋巴结转移模型的建立及其转移特征观察
World J Surg Oncol. 2015 Mar 14;13:109. doi: 10.1186/s12957-015-0528-3.
5
Vascular endothelial growth factor-C expression and its relationship to pelvic lymph node status in invasive cervical cancer.血管内皮生长因子-C在浸润性宫颈癌中的表达及其与盆腔淋巴结状态的关系。
Br J Cancer. 2001 Jul 6;85(1):93-7. doi: 10.1054/bjoc.2001.1846.
6
Quantification of vascular endothelial growth factor-C and its receptor-3 messenger RNA with real-time quantitative polymerase chain reaction as a predictor of lymph node metastasis in human colorectal cancer.采用实时定量聚合酶链反应对血管内皮生长因子-C及其受体-3信使核糖核酸进行定量分析，作为人类结直肠癌淋巴结转移的预测指标。
Surgery. 2003 Mar;133(3):300-8. doi: 10.1067/msy.2003.45.
7
Expression of VEGF-C and VEGF-D as significant markers for assessment of lymphangiogenesis and lymph node metastasis in non-small cell lung cancer.VEGF-C 和 VEGF-D 的表达作为非小细胞肺癌淋巴管生成和淋巴结转移评估的重要标志物。
Anat Rec (Hoboken). 2010 May;293(5):802-12. doi: 10.1002/ar.21096.
8
Combination of IL-24 and cisplatin inhibits angiogenesis and lymphangiogenesis of cervical cancer xenografts in a nude mouse model by inhibiting VEGF, VEGF-C and PDGF-B.白细胞介素-24与顺铂联合使用通过抑制血管内皮生长因子（VEGF）、血管内皮生长因子-C（VEGF-C）和血小板衍生生长因子-B（PDGF-B），抑制裸鼠模型中宫颈癌异种移植瘤的血管生成和淋巴管生成。
Oncol Rep. 2015 May;33(5):2468-76. doi: 10.3892/or.2015.3853. Epub 2015 Mar 16.
9
[Study of the correlation between HER-2 gene and lymphangiogenesis and their prognostic significance in human breast cancer].[HER-2基因与淋巴管生成的相关性及其在人乳腺癌中的预后意义研究]
Zhonghua Yi Xue Za Zhi. 2007 Jan 16;87(3):155-60.
10
The balance of VEGF-C and VEGFR-3 mRNA is a predictor of lymph node metastasis in non-small cell lung cancer.VEGF-C与VEGFR-3信使核糖核酸的平衡是预测非小细胞肺癌淋巴结转移的一个指标。
Br J Cancer. 2006 Jul 3;95(1):75-9. doi: 10.1038/sj.bjc.6603209. Epub 2006 Jun 6.

引用本文的文献

1
Cottontail Rabbit Papillomavirus (CRPV) Related Animal Models for Head and Neck Cancer Research: A Comprehensive Review of the Literature.棉尾兔乳头瘤病毒（CRPV）相关动物模型在头颈部癌症研究中的应用：文献综述。
Viruses. 2024 Oct 31;16(11):1722. doi: 10.3390/v16111722.
2
Tumor microenvironment promotes lymphatic metastasis of cervical cancer: its mechanisms and clinical implications.肿瘤微环境促进宫颈癌的淋巴转移：其机制及临床意义。
Front Oncol. 2023 May 10;13:1114042. doi: 10.3389/fonc.2023.1114042. eCollection 2023.
3
Rabbit VX2 Liver Tumor Model: A Review of Clinical, Biology, Histology, and Tumor Microenvironment Characteristics.

本文引用的文献

1
Systematic review of model-based cervical screening evaluations.基于模型的子宫颈癌筛查评估的系统评价
BMC Cancer. 2015 May 1;15:334. doi: 10.1186/s12885-015-1332-8.
2
The prognostic significance of histologic type in early stage cervical cancer - A multi-institutional study.早期宫颈癌组织学类型的预后意义——一项多机构研究。
Gynecol Oncol. 2015 Jun;137(3):474-8. doi: 10.1016/j.ygyno.2015.02.005. Epub 2015 Feb 10.
3
A systematic review of cervical cancer incidence and mortality in the Pacific Region.对太平洋地区宫颈癌发病率和死亡率的系统评价。
兔VX2肝肿瘤模型：临床、生物学、组织学及肿瘤微环境特征综述
Front Oncol. 2022 May 10;12:871829. doi: 10.3389/fonc.2022.871829. eCollection 2022.
4
Destructive effect of HIFU on rabbit embedded endometrial carcinoma tissues and their vascularities.高强度聚焦超声对兔移植性子宫内膜癌组织及其血管的破坏作用。
Oncotarget. 2017 Mar 21;8(12):19577-19591. doi: 10.18632/oncotarget.14751.
Asian Pac J Cancer Prev. 2014;15(21):9433-7. doi: 10.7314/apjcp.2014.15.21.9433.
4
Worldwide incidence of cervical lesions: a systematic review.全球宫颈病变发病率：一项系统综述。
Epidemiol Infect. 2015 Jan;143(2):225-41. doi: 10.1017/S0950268814001356. Epub 2014 May 30.
5
Associations of nm23H1, VEGF-C, and VEGF-3 receptor in human prostate cancer.人前列腺癌中nm23H1、血管内皮生长因子-C（VEGF-C）及血管内皮生长因子-3受体（VEGF-3受体）的相关性
Molecules. 2014 May 23;19(5):6851-62. doi: 10.3390/molecules19056851.
6
Improved survival with bevacizumab in advanced cervical cancer.贝伐珠单抗治疗晚期宫颈癌可提高生存率。
N Engl J Med. 2014 Feb 20;370(8):734-43. doi: 10.1056/NEJMoa1309748.
7
Sentinel lymph node evaluation in women with cervical cancer.宫颈癌女性患者的前哨淋巴结评估
J Minim Invasive Gynecol. 2014 Jul-Aug;21(4):540-5. doi: 10.1016/j.jmig.2013.12.095. Epub 2014 Jan 7.
8
VEGF signaling in cancer treatment.血管内皮生长因子信号通路在癌症治疗中的作用。
Curr Pharm Des. 2014;20(17):2834-42. doi: 10.2174/13816128113199990590.
9
VEGF-c expression in an in vivo model of orthotopic endometrial cancer and retroperitoneal lymph node metastasis.VEGF-c在原位子宫内膜癌及腹膜后淋巴结转移体内模型中的表达
Reprod Biol Endocrinol. 2013 May 21;11:49. doi: 10.1186/1477-7827-11-49.
10
Clinicopathological correlation and prognostic significance of VEGF-A, VEGF-C, VEGFR-2 and VEGFR-3 expression in colorectal cancer.结直肠癌中 VEGF-A、VEGF-C、VEGFR-2 和 VEGFR-3 表达的临床病理相关性及预后意义。
Cancer Genomics Proteomics. 2013 Mar-Apr;10(2):55-67.