de Novaes Oliveira Rafael, Iamamoto Keila, Silva Maria Luana Cristiny Rodrigues, Achkar Samira Maria, Castilho Juliana Galera, Ono Ekaterina Durymanova, Lobo Renata Spinelli Vaz, Brandão Paulo Eduardo, Carnieli Pedro, Carrieri Maria Luiza, Kotait Ivanete, Macedo Carla Isabel
Seção de Diagnóstico da Raiva, Instituto Pasteur, Av. Paulista, 393-Cerqueira César, São Paulo, SP, CEP 01311-000, Brazil,
Arch Virol. 2014 Oct;159(10):2615-20. doi: 10.1007/s00705-014-2121-4. Epub 2014 May 27.
Eastern equine encephalitis is a viral zoonosis that exhibits complex distribution and epidemiology, and greater importance should be given to this disease by the public-health authorities. In Brazil, although eastern equine encephalitis virus (EEEV) has been identified in vectors and antibodies are sometimes detected in horses and humans, there have been no records of equine encephalitis in horses caused by this virus during the last 24 years. This study describes eighteen cases of eastern equine encephalomyelitis that occurred in six Brazilian states between 2005 and 2009. Viral RNA was identified using semi-nested RT-PCR to detect members of the genus Alphavirus, and by genetic sequencing. The gene encoding NSP1 was partially amplified, and after genetic sequencing, eighteen sequences were generated. All eighteen strains were classified as belonging to lineage III of American EEEV. These findings could be an indication of the importance of this virus in animal and human public health.
东部马脑炎是一种病毒性人畜共患病，其分布和流行病学情况复杂，公共卫生当局应更加重视这种疾病。在巴西，尽管在病媒中已鉴定出东部马脑炎病毒（EEEV），并且有时在马和人类中检测到抗体，但在过去24年中没有关于该病毒引起马的马脑炎的记录。本研究描述了2005年至2009年间在巴西六个州发生的18例东部马脑脊髓炎病例。使用半巢式RT-PCR检测甲病毒属成员并通过基因测序鉴定病毒RNA。对编码NSP1的基因进行了部分扩增，基因测序后产生了18个序列。所有18个菌株都被归类为属于美国EEEV的谱系III。这些发现可能表明这种病毒在动物和人类公共卫生中的重要性。
