Kang Ju-Gyeong, Wang Ping-yuan, Hwang Paul M
Center for Molecular Medicine, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA.
Center for Molecular Medicine, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA.
Methods Enzymol. 2014;542:209-21. doi: 10.1016/B978-0-12-416618-9.00011-X.
There is growing evidence in the basic science field that aberrant metabolism plays an important role in tumorigenesis. Therefore, it is imperative to perform investigations in human subjects to determine (1) whether the metabolic observations made in model systems are applicable to humans; and (2) if indeed applicable, whether the metabolic alterations are clinically significant for cancer development. As an initial step, here we describe methods for measuring the mitochondrial metabolism of blood lymphocytes and skeletal muscle myoblasts that can be obtained from human subjects.
基础科学领域越来越多的证据表明，异常代谢在肿瘤发生中起着重要作用。因此，必须在人体中进行研究，以确定：（1）在模型系统中观察到的代谢情况是否适用于人类；（2）如果确实适用，代谢改变对癌症发展是否具有临床意义。作为第一步，我们在此描述从人体获取的血液淋巴细胞和骨骼肌成肌细胞线粒体代谢的测量方法。
