International Centre for Terrestrial Antarctic Research, School of Science, University of Waikato, Hamilton, New Zealand.
FEMS Microbiol Ecol. 2014 Aug;89(2):451-64. doi: 10.1111/1574-6941.12358. Epub 2014 Jun 17.
A unique collection of Antarctic aquatic environments (meltwater ponds) lies in close proximity on the rock and sediment-covered undulating surface of the McMurdo Ice Shelf, near Bratina Island (Victoria Land, Antarctica). During the 2009-10 mid-austral summer, sets of discrete water samples were collected across the vertical geochemical gradients of five meltwater ponds (Egg, P70E, Legin, Salt and Orange) for geochemical and microbial community structure analysis. Bacterial DNA fingerprints (using Automated Ribosomal Intergenic Spacer Analysis) statistically clustered communities within ponds based on anosim (R = 0.766, P = 0.001); however, one highly stratified pond (Egg) had two distinct depth-related bacterial communities (R = 0.975, P = 0.008). 454 pyrosequencing at three depths within Egg also identified phylum level shifts and increased diversity with depth, Bacteroidetes being the dominant phyla in the surface sample and Proteobacteria being dominant in the bottom two depths. best analysis, which attempts to link community structure and the geochemistry of a pond, identified conductivity and pH individually, and to a lesser extent Ag(109) , NO2 and V(51) as dominant influences to the microbial community structure in these ponds. Increasing abundances of major halo-tolerant OTUs across the strong conductivity gradient reinforce it as the primary driver of community structure in this study.
在麦克默多冰架（南极洲维多利亚地的布拉蒂纳岛附近）起伏不平的岩石和沉积物覆盖表面上，有一系列独特的南极水生环境（融水池塘）紧密相邻。在 2009-10 年的中澳大利亚夏季，在五个融水池塘（Egg、P70E、Legin、Salt 和 Orange）的垂直地球化学梯度上采集了多组离散水样，用于地球化学和微生物群落结构分析。细菌 DNA 指纹图谱（使用自动核糖体基因间隔区分析）根据anosim（R = 0.766，P = 0.001）对池塘内的群落进行了统计聚类；然而，一个高度分层的池塘（Egg）有两个不同的与深度相关的细菌群落（R = 0.975，P = 0.008）。在 Egg 内的三个深度进行的 454 焦磷酸测序也确定了门水平的转移，并随着深度的增加而增加了多样性，Bacteroidetes 是表面样本中的主要门，而 Proteobacteria 是底部两个深度的主要门。最佳分析试图将池塘的群落结构和地球化学联系起来，单独确定了电导率和 pH 值，并且在较小程度上确定了 Ag(109)、NO2 和 V(51) 对这些池塘微生物群落结构的主要影响。主要耐卤生 OTUs 的丰度在强电导率梯度上的增加加强了它作为本研究中群落结构的主要驱动因素。
