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1
Manoyl oxide (13R), the biosynthetic precursor of forskolin, is synthesized in specialized root cork cells in Coleus forskohlii.毛喉素氧化物（13R），即福斯高林的生物合成前体，在毛喉鞘蕊花的特化根皮细胞中合成。
Plant Physiol. 2014 Mar;164(3):1222-36. doi: 10.1104/pp.113.228429. Epub 2014 Jan 30.
2
1.55Å-resolution structure of ent-copalyl diphosphate synthase and exploration of general acid function by site-directed mutagenesis.内贝壳杉烯二磷酸合酶的1.55埃分辨率结构及通过定点突变对一般酸功能的探索。
Biochim Biophys Acta. 2014 Jan;1840(1):184-90. doi: 10.1016/j.bbagen.2013.09.004. Epub 2013 Sep 12.
3
Gene discovery of modular diterpene metabolism in nonmodel systems.非模式系统中模块化二萜代谢的基因发现。
Plant Physiol. 2013 Jun;162(2):1073-91. doi: 10.1104/pp.113.218347. Epub 2013 Apr 23.
4
A single residue change leads to a hydroxylated product from the class II diterpene cyclization catalyzed by abietadiene synthase.一个单一的残基变化导致了由 abietadiene 合酶催化的 II 类二萜环化反应的羟化产物。
Org Lett. 2012 Dec 7;14(23):5828-31. doi: 10.1021/ol3026022. Epub 2012 Nov 20.
5
Toward a biosynthetic route to sclareol and amber odorants.通向合成雪松香和琥珀香气息的生物合成途径。
J Am Chem Soc. 2012 Nov 21;134(46):18900-3. doi: 10.1021/ja307404u. Epub 2012 Nov 7.
6
A diterpene synthase from the clary sage Salvia sclarea catalyzes the cyclization of geranylgeranyl diphosphate to (8R)-hydroxy-copalyl diphosphate.一种来自快乐鼠尾草 Salvia sclarea 的二萜合酶催化香叶基香叶基二磷酸环化为(8R)-羟基-牻牛儿基二磷酸。
Phytochemistry. 2013 Jul;91:93-9. doi: 10.1016/j.phytochem.2012.07.019. Epub 2012 Sep 6.
7
Discovery and functional characterization of two diterpene synthases for sclareol biosynthesis in Salvia sclarea (L.) and their relevance for perfume manufacture.发现并功能表征薰衣草（Salvia sclarea (L.)）中用于合成鼠尾草烯醇的两种二萜合酶及其与香水制造的相关性。
BMC Plant Biol. 2012 Jul 26;12:119. doi: 10.1186/1471-2229-12-119.
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Characterization of two genes for the biosynthesis of the labdane diterpene Z-abienol in tobacco (Nicotiana tabacum) glandular trichomes.鉴定烟草（Nicotiana tabacum）腺毛中二萜类化合物 Z-贝壳杉烯醇生物合成的两个基因。
Plant J. 2012 Oct;72(1):1-17. doi: 10.1111/j.1365-313X.2012.05068.x. Epub 2012 Jul 17.
9
Bifunctional cis-abienol synthase from Abies balsamea discovered by transcriptome sequencing and its implications for diterpenoid fragrance production.通过转录组测序发现的白杄双功能顺式-法呢烯合酶及其对二萜类香料生产的影响。
J Biol Chem. 2012 Apr 6;287(15):12121-31. doi: 10.1074/jbc.M111.317669. Epub 2012 Feb 15.
10
Insights into diterpene cyclization from structure of bifunctional abietadiene synthase from Abies grandis.从大果冷杉双功能贝壳杉烯合酶的结构探讨二萜环化
J Biol Chem. 2012 Feb 24;287(9):6840-50. doi: 10.1074/jbc.M111.337592. Epub 2012 Jan 4.

基于植物激素生物合成中内贝壳杉烯二磷酸合酶的机理分析的新型产物化学

Novel product chemistry from mechanistic analysis of ent-copalyl diphosphate synthases from plant hormone biosynthesis.

作者信息

Potter Kevin, Criswell Jared, Zi Jiachen, Stubbs Alisha, Peters Reuben J

机构信息

Dept. Biochemistry, Biophysics & Molecular Biology, Iowa State University, Ames, IA 50011 (USA).

出版信息

Angew Chem Int Ed Engl. 2014 Jul 7;53(28):7198-202. doi: 10.1002/anie.201402911. Epub 2014 May 23.

DOI:10.1002/anie.201402911
PMID:24862907
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4113509/
Abstract

An active-site water molecule coordinated by conserved histidine and asparagine residues seems to serve as the catalytic base in all ent-copalyl diphosphate synthases (CPSs). When these residues are substituted by alanine, the mutant CPSs produce stereochemically novel ent-8-hydroxy-CPP. Given the requisite presence of CPSs in all land plants for gibberellin phytohormone biosynthesis, such plasticity presumably underlies the observed extensive diversification of the resulting labdane-related diterpenoids.

摘要

由保守的组氨酸和天冬酰胺残基配位的活性位点水分子似乎在所有内贝壳杉烯二磷酸合酶（CPS）中充当催化碱。当这些残基被丙氨酸取代时，突变的CPS会产生立体化学上新颖的内-8-羟基-CPP。鉴于所有陆地植物中CPS对于赤霉素植物激素生物合成的必要存在，这种可塑性大概是所观察到的由此产生的与半日花烷型二萜广泛多样化的基础。