Immunology and Virology Section, Laboratory of Immunology, National Eye Institute, National Institutes of Health, Bethesda, MD, United States.
Department of Pathology, Johns Hopkins University, School of Medicine, Baltimore, MD, United States.
J Neuroimmunol. 2014 Jul 15;272(1-2):42-50. doi: 10.1016/j.jneuroim.2014.05.002. Epub 2014 May 10.
The coronavirus, mouse hepatitis virus (MHV), JHM strain induces a biphasic disease in BALB/c mice that consists of an acute retinitis followed by progression to a chronic retinal degeneration with autoimmune reactivity. Retinal degeneration resistant CD-1 mice do not develop either the late phase or autoimmune reactivity. A mouse RPE/choroid DNA expression library was screened using sera from virus infected BALB/c mice. Two clones were identified, villin-2 protein and α-fodrin protein. α-Fodrin protein was used for further analysis and western blot reactivity was seen only in sera from virus infected BALB/c mice. CD4 T cells were shown to specifically react with MHV antigens and with α-fodrin protein. These studies clearly identified both antibody and CD4 T cell reactivities to α-fodrin in sera from virus infected, retinal degenerative susceptible BALB/c mice.
冠状病毒、鼠肝炎病毒 (MHV) JHM 株在 BALB/c 小鼠中引起双相疾病，包括急性视网膜炎，随后进展为慢性视网膜变性伴自身免疫反应。视网膜变性抗性 CD-1 小鼠既不会发生后期阶段也不会发生自身免疫反应。使用来自病毒感染的 BALB/c 小鼠的血清筛选了小鼠 RPE/脉络膜 DNA 表达文库。鉴定了两个克隆，即绒毛蛋白 2 蛋白和α-肌动蛋白蛋白。α-肌动蛋白蛋白用于进一步分析，仅在来自病毒感染的 BALB/c 小鼠的血清中观察到 Western blot 反应性。显示 CD4 T 细胞特异性地与 MHV 抗原和α-肌动蛋白蛋白反应。这些研究清楚地鉴定了病毒感染、视网膜变性易感 BALB/c 小鼠血清中的抗体和 CD4 T 细胞对α-肌动蛋白的反应性。
