Mu Lixian, Tang Jing, Liu Han, Shen Chuanbin, Rong Mingqiang, Zhang Zhiye, Lai Ren
Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China; and Kunming College of Life Science, University of Chinese Academy of Sciences, Beijing, China.
Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China; and.
FASEB J. 2014 Sep;28(9):3919-29. doi: 10.1096/fj.13-248476. Epub 2014 May 27.
Although it is well known that wound healing proceeds incredibly quickly in urodele amphibians, such as newts and salamanders, little is known about skin-wound healing, and no bioactive/effector substance that contributes to wound healing has been identified from these animals. As a step toward understanding salamander wound healing and skin regeneration, a potential wound-healing-promoting peptide (tylotoin; KCVRQNNKRVCK) was identified from salamander skin of Tylototriton verrucosus. It shows comparable wound-healing-promoting ability (EC50=11.14 μg/ml) with epidermal growth factor (EGF; NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR) in a murine model of full-thickness dermal wound. Tylotoin directly enhances the motility and proliferation of keratinocytes, vascular endothelial cells, and fibroblasts, resulting in accelerated reepithelialization and granulation tissue formation in the wound site. Tylotoin also promotes the release of transforming growth factor β1 (TGF-β1) and interleukin 6 (IL-6), which are essential in the wound healing response. Gene-encoded tylotoin secreted in salamander skin is possibly an effector molecule for skin wound healing. This study may facilitate understanding of the cellular and molecular events that underlie quick wound healing in salamanders.
虽然众所周知，诸如蝾螈和火蜥蜴等有尾两栖动物的伤口愈合速度极快，但关于皮肤伤口愈合的了解却很少，而且尚未从这些动物中鉴定出任何有助于伤口愈合的生物活性/效应物质。作为迈向了解蝾螈伤口愈合和皮肤再生的一步，从疣螈的蝾螈皮肤中鉴定出了一种潜在的促进伤口愈合的肽（tylotoin；KCVRQNNKRVCK）。在全层皮肤伤口的小鼠模型中，它显示出与表皮生长因子（EGF；NSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKWWELR）相当的促进伤口愈合的能力（EC50 = 11.14 μg/ml）。Tylotoin直接增强角质形成细胞、血管内皮细胞和成纤维细胞的运动性和增殖，从而加速伤口部位的上皮再形成和肉芽组织形成。Tylotoin还促进转化生长因子β1（TGF-β1）和白细胞介素6（IL-6）的释放，这两种物质在伤口愈合反应中至关重要。蝾螈皮肤中分泌的基因编码tylotoin可能是皮肤伤口愈合的效应分子。这项研究可能有助于理解蝾螈快速伤口愈合背后的细胞和分子事件。
